Figure 1.

Tissue sampled during in vitro shoot organogenesis. Internode explants from in vitro micropropagation were sampled for RNA extraction at five sequential time points. They were first placed on callus induction medium (CIM) and then on shoot induction medium (SIM). The sample times were: (A) directly after removal from parent plants and prior to placement on CIM; (B) 3 days after placement on CIM; (C) 15 days on CIM; (D) 3 days on SIM after CIM treatment; and (E) 8 days on SIM after CIM treatment.