Figure 5.

Barcode differentiation using the combined matK sequence and the spacer region of psbA-trnH. Combined barcodes were analyzed in Parthenium species, P. incanum, P. tomentosum, P. schottii, hybrid AZ101 (P. argentatum × P. tomentosum) and P. argentatum lines AZ1, AZ2, AZ3, AZ4, AZ5, AZ6, Cal6, C156, C86 and cv. 11591. UPGMA in Jukes-Cantor mode was used to construct the tree, with statistical support for tree branches evaluated by bootstrap analysis (1000 replicates), indicated above the node. Helianthus annuus was used as an outgroup.