Research article
Identification of flowering genes in strawberry, a perennial SD plant
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* Corresponding author: Timo Hytönen timo.hytonen@helsinki.fi
- † Equal contributors
1 Department of Applied Biology, PO Box 27, FIN-00014 University of Helsinki, Helsinki, Finland
2 Finnish Graduate School in Plant Biology, PO Box 56, FIN-00014 University of Helsinki, Helsinki, Finland
3 Viikki Graduate School in Biosciences, PO Box 56, FIN-00014 University of Helsinki, Helsinki, Finland
4 Horticultural Sciences Department, University of Florida, Gainesville, FL, USA
5 Institute of Biotechnology, PO Box 56, FIN-00014 University of Helsinki, Helsinki, Finland
BMC Plant Biology 2009, 9:122 doi:10.1186/1471-2229-9-122
Published: 28 September 2009Additional files
Additional file 1:
Functional classification of ESTs from EB and SD genotypes. The percentage of gene hits in different FunCat classes in two cDNA libraries prepared in this study is shown. Same gene may be classified in one or several classes. WT and EB libraries were prepared from SD and everbearing genotypes, respectively.
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Additional file 2:
Complete list of flowering time genes searched. Genes belonging to different flowering pathways are listed in separate sheets of .xls file. Homologous sequences found in Fragaria are indicated. Moreover, corresponding Rosaceae sequences were searched, if Fragaria sequence was not found. GenBank EST sequence number or Genome Database for Rosaceae contig number is given for Fragaria and Rosaceae ESTs and contigs, respectively.
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Additional file 3:
The stability of control genes used in this study. Ct values of FVE, MSI and UBI in the leaf samples collected at four leaf stage (a) and in the pooled shoot apex samples (b). Same plant material of SD and EB ('Baron Solemacher') genotypes was used than in Table 6. Panel c: Ct values of UBI in the shoot apex samples of SD and EB genotypes at different developmental stages. Values are means (± standard deviation) of two (a, b) or three (c) biological and three technical replicates. One μg of total RNA was used for cDNA synthesis for each sample. Different Ct values of UBI in shoot apex samples in figures b and c are due to different cDNA dilutions used for PCR.
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