Table 1 |
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Identification of protein spots deemed different between sap from control and P. sojae elicitor or B. japonicum treated plants |
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|
Spot No |
Annotation [organism] |
TIGR TA ID |
Number of peptides matched |
MASCOT Score |
Observed pI/Theoretical pI |
Observed MW/Theoretical MW (kD) |
Signal peptide |
Response to treatmenta |
|
|
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|
1, 2, 7, 8 |
Trypsin inhibitor A precursor [Glycine max (Soybean)] |
TA42304_3847 |
5 |
147 |
5.0/4.6 |
10.9/20.1 |
Yes |
|
|
3 |
Stem 31 kDa glycoprotein precursor [Glycine max (Soybean)] |
AI736946 |
4 |
144 |
8.5/8.5 |
15.7/29.3 |
Likely |
|
|
4 |
Stem 31 kDa glycoprotein precursor [Glycine max (Soybean)] |
TA42145_3847 |
10 |
385 |
6.1/5.9 |
27.6/25.3 |
Yes |
|
|
5 |
Gamma-glutamyl hydrolase precursor [Glycine max (Soybean)] |
TA43569_3847 |
7 |
482 |
7.9/6.3 |
34.6/35.3 |
Yes |
Bj-- |
|
6 |
CPRD14 protein -- Cinnamoyl alcohol dehydrogenase [Vigna unguiculata (cowpea)] |
TA46458_3847 |
10 |
537 |
7.9/5.9 |
34.6/35.8 |
No |
|
|
9 |
Trypsin inhibitor A precursor [Glycine max (Soybean)] |
TA42304_3847 |
5 |
247 |
4.5/4.6 |
10.4/20.1 |
Yes |
|
|
10, 11, 23 |
Stem 31 kDa glycoprotein precursor [Glycine max (Soybean)] |
TA41989_3847 |
13 |
705 |
8.7/8.5 |
27.0/29.3 |
Likely |
|
|
12 |
Acid phosphatase precursor [Phaseolus vulgaris (Kidney bean) (French bean)] |
TA42402_3847 |
8 |
392 |
7.0/5.8 |
63.6/49.7 |
Yes |
Bj-- |
|
13, 14 |
Stem 31 kDa glycoprotein precursor [Glycine max (Soybean)] |
TA42145_3847 |
15 |
1042 |
6.4/5.9 |
30.9/25.3 |
Yes |
|
|
15 |
Xyloglucan endotransglycosylase [Malus domestica (Apple) (Malus sylvestris)] |
TA43786_3847 |
5 |
205 |
8.4/6.8 |
32.3/31.9 |
No |
Bj +++ |
|
16 |
Cyclophilin [Kandelia candel] |
TA43384_3847 |
3 |
89 |
7.0/8.7 |
15.3/18.3 |
No |
Bj++ |
|
17 |
Cytosolic malate dehydrogenase [Glycine max (Soybean)] |
TA43756_3847 |
4 |
173 |
6.8/6.3 |
24.6/35.5 |
No |
Bj++ |
|
18 |
20S proteasome beta subunit C-1 [Arabidopsis thaliana] |
TA46641_3847 |
2 |
87 |
5.1/5.3 |
21.0/22.8 |
No |
Bj++ |
|
19 |
Gamma-glutamyl hydrolase precursor [Glycine max (Soybean)] |
TA43569_3847 |
6 |
315 |
7.6/6.1 |
37.8/37.7 |
Yes |
|
|
20 |
Cationic peroxidase 1, putative, expressed [Oryza sativa (japonica cultivar-group)] |
TA64044_3847 |
2 |
136 |
7.0/5.34 |
10.9/27.0 |
Yes |
Bj-- |
|
21, 22 |
Beta-amylase [Glycine max (Soybean)] |
TA44402_3847 |
4 |
203 |
6.8/5.4 |
24.6/56.2 |
No |
|
|
24 |
Putative globulin/legumin [Arabidopsis thaliana] |
TA42556_3847 |
4 |
213 |
4.7/5.8 |
37.8/38.3 |
No |
|
|
25 |
Cationic peroxidase 1 precursor [Arachis hypogaea (Peanut)] |
TA59475_3847 |
2 |
134 |
7.0/8.1 |
38.7/31.2 |
Yes |
Ps++ |
|
26 |
Putative subtilisin precursor [Glycine max (Soybean)] |
TA41917_3847 |
3 |
275 |
7.3/6.6 |
97.8/80.0 |
Yes |
Ps++ |
|
|
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|
a Increase (+) or decrease (-) of spot intensity in response to B. japonicum or P. sojae elicitor treatment is indicated. |
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|
Subramanian et al. BMC Plant Biology 2009 9:119 doi:10.1186/1471-2229-9-119 |
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