Carboxy-terminal substitutions do not influence the structural stability of the enzyme in assays performed in vitro. Thermal inactivation of Rubisco purified from wild type (○) and the D470P/T471A/I472M/K474T quadruple mutant (●). Rubisco enzymes were incubated at each temperature for 10 min, and then assayed for RuBP carboxylase activity at 25°C. Activities were normalized to the specific activities measured after the 35°C incubation (wild type, 1.7 μmol/min/mg; D470P/T471A/I472M/K474T, 1.2 μmol/min/mg).
Satagopan and Spreitzer BMC Plant Biology 2008 8:85 doi:10.1186/1471-2229-8-85