Table 1

Expectations of results from further sequencing of a blackberry (Rubus L.) EST library.

Subset sequenced

Multiplication rate

Whole library

3884

18,432

Number of clones

77%

3000

14,237

Number of high quality sequences

22%

673

3,132

Number of SSR primer pairs to test in parental screens

30%

202

940

Number of primer pairs detecting polymorphisms between two genotypes

× 1.9

384

1,786

Number of polymorphisms to try to map

Expectations are based on results from this study of the percentage of high quality sequences obtained, the percentage of those sequences which contained SSRs, the percentage of primer pairs selected to test, the percentage of SSRs that detected polymorphisms, and the average number of polymorphic products generated from each.

Lewers et al. BMC Plant Biology 2008 8:69   doi:10.1186/1471-2229-8-69