Figure 6.

Accumulation of ER at the point of contact. ER visualized in the cortical cytoplasm underlying the outer epidermal cell wall in a cotyledon of A. thaliana expressing GFP-KKXX [71]. The surface of the epidermal cell was touched with a glass microneedle at time 0:00 at the position marked by the asterisk in B. Images A-F are projections of five optical sections taken at the times indicated, with the image in A being collected about 2 minutes before the cotyledon was touched with the needle. ER began to aggregate beneath the tip of the needle 3–4 minutes after touching the epidermal cell surface. Strands of diffuse fluorescence form near the contact sites and a cloud of bright fluorescence forms and expands. Bar = 10 μm

Hardham et al. BMC Plant Biology 2008 8:63   doi:10.1186/1471-2229-8-63
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