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Open Access Research article

Global expression profiling in leaves of free-growing aspen

Andreas Sjödin1, Kirsten Wissel13, Max Bylesjö2, Johan Trygg2 and Stefan Jansson1*

Author Affiliations

1 Um eå Plant Science Centre, Department of Plant Physiology, Um eå University, SE-901 87 Um eå, Sweden

2 Research Group for Chemometrics, Department of Chemistry, Um eå University, SE-901 87 Um eå, Sweden

3 Department of Otolaryngology, Medical University of Hannover, Carl-Neuberg Str. 1, D-30625 Hannover, Germany

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BMC Plant Biology 2008, 8:61  doi:10.1186/1471-2229-8-61

Published: 23 May 2008

Abstract

Background

Genomic studies are routinely performed on young plants in controlled environments which is very different from natural conditions. In reality plants in temperate countries are exposed to large fluctuations in environmental conditions, in the case of perennials over several years. We have studied gene expression in leaves of a free-growing aspen (Populus tremula) throughout multiple growing seasons

Results

We show that gene expression during the first month of leaf development was largely determined by a developmental program although leaf expansion, chlorophyll accumulation and the speed of progression through this program was regulated by the temperature. We were also able to define "transcriptional signatures" for four different substages of leaf development. In mature leaves, weather factors were important for gene regulation.

Conclusion

This study shows that multivariate methods together with high throughput transcriptional methods in the field can provide additional, novel information as to plant status under changing environmental conditions that is impossible to mimic in laboratory conditions. We have generated a dataset that could be used to e.g. identify marker genes for certain developmental stages or treatments, as well as to assess natural variation in gene expression.