Additional file 4.
Example of a grape EST contigs matched by multiple MPSS signatures. One of the EST contigs with 3 MPSS signatures matches is shown. This contig (CTG1027770) has similarity to "putative transcription factor BTF3-like mRNA". Panel A shows all the MPSS signatures identified in this contig, with the abundance level and its coordinate on the contig. Panel B displays the sequence of this contig and all the sense MPSS signatures from panel A are indicated in blue. Uppercase letters indicate the predicted ORF, while lowercase letters indicate the predicted UTRs. The position of the most abundant signature (#2) is consistent with the most-3' DpnII site, the position measured by MPSS. Other signatures may result from signatures resulting from other transcripts, alternative polyadenylation or incomplete digestion during the construction of the MPSS library.
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Iandolino et al. BMC Plant Biology 2008 8:53 doi:10.1186/1471-2229-8-53