Different subcellular localizations and functions of Arabidopsis myosin VIII

Additional file 1:

Three dimension reconstruction of lateral root of GFP-ATM1(IQ-tail) expressing plant. Rotation of the 3D image shows that the ATM1-dots are scattered in the cells at different optic planes.

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Additional file 2:

BFA and FM4-64 treatment to GFP expressing seedlings. Five day old seedlings expressing GFP were subjected to BFA and FM4-64 treatment as described for ATM1 expressing plants. A. GFP B. FM4-64. C. overlay of A and B. Scale bar 5 μm, 1 optic section.

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Additional file 3:

Time lapse movie of RFP-ATM1 (IQ-tail) expressed in an abaxial leaf epidermal cell of N. benthamiana. Images were acquired every 1.3 second and are run at 8 frames per second. It is shown that ATM1 dots are mostly stationary but rarely motile dots are seen.

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Additional file 4:

Expression profiles of the four members of myosin VIII in the different plant organs as determined by Genevestigator.

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Additional file 5:

Time lapse movie of GFP-ARA7 and RFP-ATM1(IQ-tail) expressed in an abaxial leaf epidermal cell of N. benthamiana. Images were acquired every 1.3 second and are run at 8 frames per second. It is shown that while GFP-ARA7 labeled organelles are moving in the cytoplasm, ATM1 dots are stationary at the plasma membrane. The difference between the plasma membrane depicted by the ATM1 labeling and the rapidly moving cytoplasm and organelles is emphasized in this movie. Scale bar 5 μm.

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Additional file 6:

Time lapse movie of GFP-ATM1(IQ-tail) and DsRed-FYVE expressed in an abaxial leaf epidermal cell of N. benthamiana. Images were acquired every 1.3 second and are run at 8 frames per second. Motile FYVE labeled organelles are shown, some of them associated with ATM1. The speed of movement is sometime faster than the time of switch between the two lasers. This is seen by the separation of the green and magenta dots in some of the frames. Scale bar 5 μm.

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Additional file 7:

Time lapse movie of GFP-ATM1(IQ-tail) and DsRed-FYVE expressed in an abaxial leaf epidermal cell of N. benthamiana. Images were acquired every 1.3 second and are run at 8 frames per second. Motile FYVE labeled organelles are shown, but one of them which is completely surrounded by GFP-ATM1 is stationary.

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Additional file 8:

Time lapse movie of ARA6-GFP expressed in an abaxial leaf epidermal cell of N. benthamiana. Images were acquired every 1.3 second and are run at 8 frames per second. Motile ARA6-GFP labeled organelles are shown.

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Additional file 9:

Time lapse movie of ARA6-GFP and RFP-ATM1(IQ-tail) expressed in an abaxial leaf epidermal cell of N. benthamiana. Images were acquired every 1.3 second and are run at 8 frames per second. It is shown that only ARA6-GFP organelles free from RFP-ATM1 are motile while all the double labeled organelles are motionless. Scale bar 5 μm.

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