Research article
Association of six YFP-myosin XI-tail fusions with mobile plant cell organelles
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* Corresponding author: Maureen R Hanson mrh5@cornell.edu
1 Department of Molecular Biology and Genetics, 321 Biotechnology Building, Cornell University, Ithaca, NY 14853, USA
2 Bitplane AG, Badenerstrasse 682, CH-8048, Zurich, Switzerland
BMC Plant Biology 2007, 7:6 doi:10.1186/1471-2229-7-6
Published: 9 February 2007Additional files
Peroxisome movement in onion cells visualized with a YFP::catalase2 construct. Onion cells were transiently transformed by bombardment with YFP::catalase2 DNA, and expression was observed 24 h later on a confocal microscope. Time series were collected and the movie displays the pictures with a 20-fold acceleration.
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Peroxisome movement in tobacco leave cells visualized with a YFP::catalase2 construct. N. benthamiana leaves were infiltrated with Agrobacterium carrying a YFP::catalase2 construct. Expression was observed 48 h later on a confocal microscope. Time series were collected and the movie displays the pictures with a 16-fold acceleration.
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Movement of YFP::myosin XI-6-tail labeled organelles. N. benthamiana leaves were infiltrated with Agrobacterium carrying a YFP::myosin XI-6-tail construct. Expression was observed 48 h later on a confocal microscope. Time series were collected and the movie displays the pictures with a 16-fold acceleration.
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Movement of YFP::myosin XI-8-tail labeled organelles. Arabidopsis thaliana leaves were bombarded with YFP::myosin XI-8-tail DNA and expression was observed 24 h later on a confocal microscope. Time series were collected and the movie displays the pictures with a 16-fold acceleration.
Format: MOV Size: 7MB Download file
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Movement of YFP::myosin XI-15-tail labeled organelles. Onion cells were transiently transformed by bombardment with YFP::myosin XI-15-tail DNA, and expression was observed 24 h later on a confocal microscope. Time series were collected and the movie displays the pictures with a 10-fold acceleration.
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Movement of YFP::myosin XI-17-tail labeled organelles. Arabidopsis thaliana leaves were bombarded with YFP::myosin XI-17-tail DNA and expression was observed 24 h later on a confocal microscope. Time series were collected and the movie displays the pictures with a 16-fold acceleration.
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Movement of YFP::myosin XI-6-nocoil labeled organelles. N. bentamiana leaves were infiltrated with Agrobacterium carrying a YFP::myosin XI-6-nocoil construct. Expression was observed 48 h later on a confocal microscope. Time series were collected and the movie displays the pictures with an 8-fold acceleration.
Format: MOV Size: 6.3MB Download file
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