Figure 2.

Phylogenetic analysis of acetylenease-related sequences from Centaurea cDNA library. C. maculosa EST sequences with similarity to fatty acid desaturase genes were clustered with previously characterized genes from Helianthus annuus (Hean), Petroselinum crispum (Pecr) fungal elicited desaturases (ELI), and with sterol desaturasase from Arabidopsis thalania as an outgroup. CENT_UG_00643, CENT_UG_00475, and CENT_UG_00098 cluster with the characterized acetylenase from H. annuus, distinct from the remaining Δ12 desaturases (Del12), suggesting potential for acetylenase activity.