Research article

A first step in understanding an invasive weed through its genes: an EST analysis of invasive Centaurea maculosa

Amanda K Broz^1,2 , Corey D Broeckling^1,3 , Ji He⁴ , Xinbin Dai⁴ , Patrick X Zhao⁴ and Jorge M Vivanco^1,2

¹  Center for Rhizosphere Biology, Colorado State University, Fort Collins, CO 80523-1173, USA

²  Department of Horticulture and Landscape Architecture, Colorado State University, Fort Collins, CO 80523-1173, USA

³  Cell and Molecular Biology Graduate Program, Colorado State University, Fort Collins, CO 80523-1173, USA

⁴  Plant Biology Division, Samuel Roberts Noble Foundation, Ardmore, OK 73401, USA

author email corresponding author email

BMC Plant Biology 2007, 7:25doi:10.1186/1471-2229-7-25

Published:	24 May 2007

Abstract

Background

The economic and biological implications of plant invasion are overwhelming; however, the processes by which plants become successful invaders are not well understood. Limited genetic resources are available for most invasive and weedy species, making it difficult to study molecular and genetic aspects that may be associated with invasion.

Results

As an initial step towards understanding the molecular mechanisms by which plants become invasive, we have generated a normalized Expressed Sequence Tag (EST) library comprising seven invasive populations of Centaurea maculosa, an invasive aster in North America. Seventy-seven percent of the 4423 unique transcripts showed significant similarity to existing proteins in the NCBI database and could be grouped based on gene ontology assignments.

Conclusion

The C. maculosa EST library represents an initial step towards looking at gene-specific expression in this species, and will pave the way for creation of other resources such as microarray chips that can help provide a view of global gene expression in invasive C. maculosa and its native counterparts. To our knowledge, this is the first published set of ESTs derived from an invasive weed that will be targeted to study invasive behavior. Understanding the genetic basis of evolution for increased invasiveness in exotic plants is critical to understanding the mechanisms through which exotic invasions occur.