Table 2

Mutations discovered in the Nipponbare populations mutagenized with either 1.5% EMS or 1 mM Az-15 mM MNU
Target
 Nucleotide Change
 Amplicon Position
 Effecta
 Population
 Stock





EMS
 Az-MNU
Os1433
 T->C
 270
 L139P+
 X
TJ_0516

T->A
 304
 D150E
 X
TJ_0521

G->A
 326
 A158T+
 X
TJ_0160

A->G
 332
 K160E
 X
TJ_0160

C->T
 595
 S194F+
 X
TJ_0024

G->T
 950
 intron
 X
TJ_0042

C->T
 1144
 intron
X
 TT_0028

G->A
 1314
 L213=
 X
TJ_0478
OsBZIP
 C->T
 141
 L46F
X
 TT_0370

G->A
 475
 S157N
X
 TT_0846

G->A
 530
 E175=
 X
TJ_0747

G->A
 574
 G190E
X
 TT_0267

G->A
 640
 G212D
X
 TT_0150

G->A
 947
 E314=
X
 TT_0781

G->A
 1012
 intron
X
 TT_0150

C->T
 1083
 L330=
 X
TJ_0504
OsCALS8R
 G->A
 93
 R291=
 X
TJ_0461

C->T
 288
 V356=
 X
TJ_0521

A->T
 458
 Y413F+
X
 TT_0314

A->G
 574
 I452V
X
 TT_0044

C->T
 643
 L475=
X
 TT_0145

G->A
 843
 E541=
 X
TJ_0143
OsDREB
 G->A
 203
 W21*
X
 TT_0320

G->A
 278
 E46=
X
 TT_0781

G->A
 318
 A60T
X
 TT_0133

G->A
 558
 D140N
X
 TT_0627

C->T
 613
 S158F
X
 TT_0593

A->C
 805
 N222T
X
 TT_0150

A->C
 805
 N222T
X
 TT_0123

G->A
 849
 V237M
 X
TJ_0230

G->A
 913
 S258N
 X
TJ_0676
OsEXTE
 C->T
 73
 intron
X
 TT_0646

A->G
 282
 E174G
X
 TT_0682

G->A
 593
 V278I
 X
TJ_0122

C->T
 651
 S297F
 X
TJ_0024

G->A
 697
 R312=
X
 TT_0426

A->G
 1024
 Q421=
X
 TT_0419

G->A
 1093
 P444=
X
 TT_0503

G->A
 1164
 C468Y
 X
TJ_0155
OsMAPK
 A->T
 254
 T331S
X
 TT_0301

T->C
 799
 G478=
X
 TT_0399

G->A
 832
 E489=
X
 TT_0437

A->T
 1181
 M606L
 X
TJ_0360
OsPITA
 G->A
 293
 D481N
X
 TT_0515

C->T
 424
 V524=
 X
TJ_0183

T->C
 724
 L624=
 X
TJ_0517

G->A
 802
 K650=
 X
TJ_0162

G->A
 872
 A674T
 X
TJ_0025
OsR1A
 C->T
 217
 T186I
X
 TT_0246

A->G
 389
 L243=
X
 TT_0257
OsRPLD1
 C->T
 252
 T371I+
X
 TT_0643
OsTPS1
 T->C
 182
 G182=
X
 TT_0898

C->T
 364
 intron
 X
TJ_0710

C->T
 521
 intron
 X
TJ_0476

A->T
 598
 Q205H+
 X
TJ_0545

G->A
 1147
 intron
 X
TJ_0415

A->T
 1458
 intron
 X
TJ_0247
Total


27
 30

a Synonymous (=), nonsense (*), and missense changes are shown, where the amino acid residue number is based on the exon-intron model for the TILLed fragment. Missense changes predicted by the program SIFT to be damaging to the encoded protein are indicated (+). Predicted damaging mutations are highlighted in bold.

Till et al. BMC Plant Biology 2007 7:19   doi:10.1186/1471-2229-7-19