Research article
Lutein is needed for efficient chlorophyll triplet quenching in the major LHCII antenna complex of higher plants and effective photoprotection in vivo under strong light
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* Corresponding author: Roberto Bassi bassi@sci.univr.it
1 Dipartimento Scientifico e Tecnologico, Università di Verona, Strada Le Grazie 15, I-37134 Verona, Italy
2 Ente per le Nuove tecnologie, l'Energia e l'Ambiente (ENEA), Unità Biotecnologie, Centro Ricerche Casaccia, C.P. 2400, Roma 00100, Italy
3 CEA/Cadarache, DSV, DEVM, Laboratoire d'Ecophysiologie de la Photosynthèse, UMR 6191 CEA-CNRS-Aix Marseille II, F-13108 Saint-Paul-lez-Durance, France
4 Laboratoire de Génétique et Biophysique des Plantes (LGBP), Département d'Ecophysiologie Végétale et Microbiologie – UMR 163 CEA-CNRS Université de la Méditerranée Aix-Marseille II, 163 Avenue de Luminy, Marseille, France
5 Institut de Biologie Physico-Chimique (IBPC), rue Pierre et Marie Curie 13, Paris, France
BMC Plant Biology 2006, 6:32 doi:10.1186/1471-2229-6-32
Published: 27 December 2006Additional files
Additional file 1:
Pigment composition of leaf tissue from WT and mutant genotypes, both dark adapted, and after light stress (1200 μmol m-2 s-1, 20' at RT).
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Additional file 2:
Photosynthetic parameters Fo, Fm, Fv/Fm and T1/2 measured on WT and lut2.1 leaves.
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Additional file 6:
Kinetics of NPQ.
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Additional file 8:
Fitting of the Soret region of LHCII 1-T spectra from WT and lut2.1.
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Additional file 3:
Pigment composition and stability to heat denaturation of fractions obtained by preparative IEF of monomeric Lhcb isolated from WT and lut2.1.
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Additional file 7:
Thermal stability of Lhcb proteins isolated from WT and lut2.1 thylakoids.
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Additional file 9:
Flat bed isoelectric focusing fractionation.
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Additional file 4:
Leaf pigment composition of 3-weeks-old WT and lut2.1 plants grown for three additional weeks LL (120 μmol m-2 s-1) or HL (1400 μmol m-2 s-1) conditions.
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Additional file 5:
Measurements of Fv/Fm ratio and chlorophyll content of WT and mutant leaves during growth at low temperature and different light conditions.
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