Figure 5.

RNA gel-blot analysis of mRNA transcripts at day 3 pre-anthesis. RNA was extracted from day 3 pre-anthesis flower buds of P. bellina (lane 1) and P. equestris (lane 2) and analyzed for PLOX1, PEPI3, PGTPS and PNADPH-dehydrogenase 1 (PNADPHDH1) expression. The blots contained 10 μg of total RNA extracted from the different mature floral buds. The ribosomal RNA signals showed that the same amounts of total RNA were loaded on each individual lane.