Table 3 |
|||
|
Quantitative real-time PCR analysis of mRNA for Gal4 and LUC genes in selected linesa. |
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|
Line# |
Fold drop in LUC activity (generations used)b |
Fold drop in gene expression |
|
|
|
|||
|
GAL4 |
LUC |
||
|
|
|||
|
169 |
1.2 (T3/T4) |
3 |
3.5 |
|
213 |
1.8(T3/T4) |
2.3 |
3.3 |
|
65L-4B |
42 (T2/T4) |
1.6 |
147 |
|
50-1 |
33 (T2/T4) |
59 |
56 |
|
36c |
82 (T2/T4) |
298 |
71 |
|
80-2 |
20 (T2/T4) |
18 |
35 |
|
|
|||
|
a All numbers indicate a fold decrease in activity or expression as plant lines progress from the T2 (or T3) to the T4 generation. b Luciferase activity measured in BASTA-selected 14 day-old seedlings by spraying 1 mM luciferin. c This is a full 35S-driven Gal4 line as shown in Fig 8. |
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|
Engineer et al. BMC Plant Biology 2005 5:9 doi:10.1186/1471-2229-5-9 |
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