Research article
New insights into the tonoplast architecture of plant vacuoles and vacuolar dynamics during osmotic stress
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* Corresponding author: Nathalie Leborgne-Castel lcastel@u-bourgogne.fr
1 UMR PME INRA/CNRS/Université de Bourgogne BP 47870, boulevard Gabriel, 21078 Dijon Cedex, France
2 Department of Molecular Biology and Genetics, 321 Biotechnology Building, Cornell University, Ithaca, NY 14853, USA
BMC Plant Biology 2005, 5:13 doi:10.1186/1471-2229-5-13
Published: 4 August 2005Additional files
Cellular tomogram through two overlapping BobTIP26-1::gfp expressing cells.
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Animation of 3-D reconstructed vacuoles. The reconstructed vacuoles from Figure 3d were rotated. Notice chloroplasts (red) on the outer sides of the vacuoles, within transvacuolar strands and the cells' peripheries.
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Cellular tomogram through a protoplast with a GFP-labeled tonoplast. The protoplast was prepared from BobTIP26-1::gfp expressing cells as previously described [17].
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Cellular tomogram through a plasmolysed cell demonstrating the intact tonoplast structure and its folding. The tonoplast fluoresces in green. Hechtian strands are not labeled. Bar = 5 μm.
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Plasmolysis-deplasmolysis cycle of cells bathed with 0.5 M sorbitol. Time is marked at the bottom left; changes of media are given at the top left (10 min: + 0.5 M sorbitol; 23.5 min: + normal MS medium). Bar = 20 μm.
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