Table 2

Results of DNA hybridizations to determine gene copy number and the number of genome equivalents represented in the arrayed BAC library.

Gene probe:

Homologous to following plant genes (e value; % amino acid identity)1:

Genome copy number:

Number of positive BAC clones from arrayed library:


SmGAI cDNA

GIBBERELLIC ACID INSENSITIVE (8e-34; 43)

1

14

SmCYP98 cDNA

CYTOCHROME P450 98A3 (1e-40; 66)

2

23

SmOTP

OXYANION TRANSLOCATION PROTEIN (1e-36; 53)

2

16

SmSHR

SHORTROOT (3e-45; 47)

1

7

SmZNF

Zn-FINGER PROTEIN (1e-69; 90)

1

7

SmChlH

Mg CHELATASE SUBUNIT H (1e-130; 85)

1

8

SmSNT

SYNTAXIN (9e-55; 54)

1

12

S. moellendorffii chloroplast fragment2

Contains:

Ribosomal protein L22 (2e-33; 56)

Ribosomal protein L2 (1e-33; 71)

Ribosomal protein S19 (2e-31; 71)

ND

207

S. moellendorffii mitochondrial fragment2

Contains NADH DEHYDROGENASE subunit 5 (6e-61; 71)

ND

0


1Gene listed is based on results of blastx using the S. moellendorffii sequence as a query against the NR database and default parameters [24].

2sequence described is part of a genomic fragment <5 kb that was used as a probe.

Wang et al. BMC Plant Biology 2005 5:10   doi:10.1186/1471-2229-5-10

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