Table 1 |
||||
|
Frequencies of gametophyte phenotypes after bombardment with various plasmids. |
||||
|
Plasmids introduced |
% colorless & GUS positivea,b |
% colorless & GUS negativea |
% green & GUS positivea |
nc |
|
|
||||
|
35S:irintCrChl+pFF19G |
88.8 (+/-1.36)d |
6.8 (+/-0.46)d |
4 (+/- 1.41)d |
1263 |
|
irintCrChl+pFF19G |
81.7 (+/-0.87) |
8.3 (+/-1.20) |
10 (+/-1.53) |
775 |
|
35S:intantiCrChl+pFF19G |
61.0 (+/-1.73) |
11.0 (+/-1.73) |
28.0 (+/-3.21) |
564 |
|
35S:antiCrChl+pFF19G |
12.0 (+/-1.53) |
0 |
88.0 (+/-1.53) |
724 |
|
antiCrChl+pFF19G |
0.7 (+/-0.33) |
0 |
99.3 (+/-0.33) |
479 |
|
35S:senseCrChlint+pFF19G |
37.7 (+/-1.12) |
6.3 (+/-0.19) |
56 (+/-1.00) |
559 |
|
35S:senseCrChl+pFF19G |
2.3 (+/-0.88) |
0 |
97.7 (+/-0.88) |
761 |
|
senseCrChl+pFF19G |
1.7 (+/-0.33) |
0 |
98.7 (+/-0.42) |
677 |
|
CrChl cDNA+pFF19G |
13.7 (+/-0.88) |
0 |
86.0 (+/-1.16) |
563 |
|
Δ35SpFF19G+ |
48.5 (+/-6.36) |
51.0 (+/-5.66) |
0.5 (+/-0.71) |
470 |
|
35SinintCrChl |
||||
|
pFF19G |
0 |
0 |
100 |
600 |
|
% with lesions & GUS positivea,b |
% with lesions & GUS negativea |
% without lesions & GUS positive |
nc |
|
|
|
||||
|
CrUrod cDNA+pFF19G |
61.0 (+/-0.71) |
20.2 (+/-0.69) |
18.9 (+/-0.43) |
774 |
|
35S:intantiCrUrod+pFF19G |
55.7 (+/-1.40) |
13.5 (+/-1.61) |
31.1 (+/- 2.25) |
472 |
|
|
||||
|
aAll gametophytes were stained 7 days after shooting; each percent represents the average of three replicates; standard error is given in parentheses. bthe differences between treatments for all pairwise comparisons are statistically significant (z>3.4, P < 0.0003) with the exception of 35S:senseCrChl+pFF19G and senseCrChl+pFF19G. ctotal number of gametophytes displaying a phenotype and scored. dthe average of 5 replicates. |
||||
|
Rutherford et al. BMC Plant Biology 2004 4:6 doi:10.1186/1471-2229-4-6 |
||||
