Table 2 |
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Substrate specificity of the recombinant F6H Both rF6Hb and rF6Hy were purified on Ni-NTA resin then Superose 12, and fractions were assayed with the indicated substrates, using the direct enzyme assay. |
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|
Substratea |
Relative enzyme activity (%) |
|
|
rF6Hb b |
rF6Hy c |
|
|
|
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|
3,7,4'-Trimethylquercetin |
100 |
100 |
|
3,7-Dimethylquercetin |
56 |
63 |
|
3,7,3',4'-Tetramethylquercetin |
11 |
21 |
|
3-Methylquercetin |
0 |
4 |
|
|
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a No F6H activity was detected with kaempferol, quercetin, myricetin, naringenin, eriodyctyol, apigenin or luteolin when used as substrate, regardless of concentration. Rhamnetin (7-O-methylquercetin, 17% at 50 μM), tamarixetin (4'-O-methylquercetin, 13 % at 50 μM) and isorhamnetin (3'-O-methylquercetin, 4 % at 50 μM) b Estimated as 0.10 pkat/mg for 100% activity and 5 μM substrate. c Estimated as 0.19 pkat/mg for 100% activity and 5 μM substrate. |
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Anzellotti and Ibrahim BMC Plant Biology 2004 4:20 doi:10.1186/1471-2229-4-20 |
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