Research article
Cloning and expression analysis of cDNAs corresponding to genes activated in cucumber showing systemic acquired resistance after BTH treatment
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* Corresponding author: Jacques Dommes J.Dommes@ulg.ac.be
1 Laboratoire de Biologie Moléculaire et de Biotechnologie Végétales, Département des Sciences de la Vie, B22, Université de Liège, B-4000 Liège/Sart Tilman, Belgium
2 Centre Wallon de Biologie Industrielle, Unité de Bioindustries, Faculté Universitaire des Sciences Agronomiques, B-5030 Gembloux, Belgium
BMC Plant Biology 2004, 4:15 doi:10.1186/1471-2229-4-15
Published: 26 August 2004Additional files
Additional File 1:
PR-8 mRNA accumulation in cucumber leaves in response to different concentrations of BTH. Total RNA was extracted from cucumber leaves 24 h and 48 h after spraying with water, 10 μM BTH, 30 μM BTH, 100 μM BTH or from untreated leaves (UT). RNA was fractionated by electrophoresis on agarose gel. Northern blot was probed with α-32P labeled PR-8 cDNA. Loading of equal amounts of RNA was confirmed by ethidium bromide (Et Br) staining.
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