Table 2

Peanut microsatellite markers showed a polymorphism among 24 genotypes

Name of locus

Primer Tm* (°C)

Annealing temperature (°C)

Number of alleles

Size range (bp)

PM3

61, 63

55

7

200–222

PM15

64, 65

55

3

200–350

PM32

63, 63

55

4

100–124

PM35

64, 63

55

3

130–140

PM36

64, 65

50

8

190–240

PM42

65, 62

50

3

200–208

PM45

65, 63

60

3

101–111

PM50

61, 63

50

8

94–224

PM53

62, 62

60

2

110–116

PM65

65, 64

55

3

320–350

PM137

62, 63

55

4

130–152

PM145

62, 63

55

3

170–180

PM183

60, 63

60

8

100–150

PM188

63, 64

55

2

106–120

PM200

62, 64

55

3

150–158

PM201

63, 64

55

3

220–228

PM204

64, 64

55

4

200–226

PM210

64, 64

55

4

180–192

PM238

63, 62

55

4

164–170

* First number is forward primer Tm and second number is reverse primer Tm.

He et al. BMC Plant Biology 2003 3:3   doi:10.1186/1471-2229-3-3