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SUMO E3 ligase AtMMS21 is required for normal meiosis and gametophyte development in Arabidopsis

BMC Plant Biology volume 14, Article number: 153 (2014) Cite this article

Abstract

Background

MMS21 is a SUMO E3 ligase that is conserved in eukaryotes, and has previously been shown to be required for DNA repair and maintenance of chromosome integrity. Loss of the Arabidopsis MMS21 causes defective meristems and dwarf phenotypes.

Results

Here, we show a role for AtMMS21 during gametophyte development. AtMMS21 deficient plants are semisterile with shorter mature siliques and abortive seeds. The mms21-1 mutant shows reduced pollen number, and viability, and germination and abnormal pollen tube growth. Embryo sac development is also compromised in the mutant. During meiosis, chromosome mis-segregation and fragmentation is observed, and the products of meiosis are frequently dyads or irregular tetrads. Several transcripts for meiotic genes related to chromosome maintenance and behavior are altered. Moreover, accumulation of SUMO-protein conjugates in the mms21-1 pollen grains is distinct from that in wild-type.

Conclusions

Thus, these results suggest that AtMMS21 mediated SUMOylation may stabilize the expression and accumulation of meiotic proteins and affect gametophyte development.

Background

The life cycle of flowering plants alternates between a prominent diploid sporophyte generation and a short-lived haploid gametophyte generation. The haploid gametophytes are derived from the haploid spores that are produced by diploid megasporocytes (female) and microsporocyte (male)parent cells [1]. During female gametophyte development, the megasporocyte undergoes meiosis to produce a tetrad of four haploid spores. Three of the spores degenerate, and one proceeds through three sequential rounds of mitotic division, forming the female gametophyte (embryo sac), which consists of seven cells with four cell types [2]. During male gametophyte development, microsporocytes undergo meiosis to form a tetrad of four haploid microspores. Each microspore undergoes two mitotic divisions to form the male gametophyte (pollen grain) consisting of a vegetative cell and two sperm cells [3]. Following pollination, the pollen grain lands on the pistil and extends a pollen tube that allows the delivery of the two sperm cells into the female gametophyte, and then gives rise to the diploid zygote to begin the sporophytic generation [4]. Female and male gametophyte development differ considerably, but at the same time share the same fundamental hallmark of being haploid organs: it is therefore logical that they might require the same basal machinery and share a number of common regulators [5].

Meiosis is a specialized cellular division that is conserved among most eukaryotes. This process is indispensable for formation of viable offspring. It consists of two rounds of chromosome segregation after a single round of DNA replication, giving rise to four haploid daughter cells. During meiosis I homologous chromosomes pair, undergo recombination and then segregate, whereas sister chromatids separate during meiosis II [6]. Recombination is initiated by the formation of SPO11-induced DNA double strand-breaks (DSBs), and DSBs in meiosis are repaired by homologous recombination [7]. Disruption of meiotic homologous recombination could result in chromosome anomalies, which could lead to mis-segregation and aneuploidy [8].

The faithful transmission of chromosomes during meiosis is essential for the survival and reproduction of flowering plants. A critical aspect of chromosome dynamics is structural maintenance of chromosome (SMC) proteins, which are responsible for sister chromatid cohesion, chromosome condensation and homologous recombination (HR) during meiosis [9, 10]. The evolutionarily conserved SMC gene family encodes members of the three complexes: the cohesin, the condensin and the SMC5/6 complex. In Arabidopsis, the cohesin complex consists of the SMC1, SMC3, SCC3, and four α-kleisin subunits: SYN1, SYN2, SYN3 and SYN4 [10]. Evidence from mutants (smc1, smc3, scc3, syn1, syn3) defective in meiosis have shown that cohesin is essential for the control of chromosome structure and many subsequent meiotic events [11–15]. The arabidopsis condensin complex consists of the SMC2, SMC4, and β-kleisin subunit CAP-D2. Data from mutants (smc2, smc4) with gametophytic defects have shown that condensin is required for chromosome condensation and segregation during mitosis, meiosis and embryo development [16–18]. In plants, knowledge about the role of SMC5/6 complex is still limited. The arabidopsis SMC5/6 complex presumably consists of SMC5, one of two alternative SMC6 proteins and four NSE(non-SMC elements) proteins (NSE1-4) [10]. It has been shown in Arabidopsis that SMC5 and SMC6 enhances sister chromatid alignment after DNA damage and thereby facilitates correct DSB repair via HR between sister chromatids [19]. Although the arabidopsis NSE1 and SMC5 are essential for seed development [19, 20], the role SMC5/6 complex in gametophyte development is still unknown.

The Arabidopsis SUMO E3 ligase AtMMS21/HPY2, a homologue of NSE2/MMS21, has been identified recently as participating in root development. Loss of AtMMS21/HPY2 function results in premature mitotic-to-endocycle transition, defective cytokinin signaling, and impaired cell cycle, leading to severe dwarfism with compromised meristems [21–23]. Recent data demonstrate that AtMMS21/HPY2 functions as a subunit of the SMC5/6 complex through its interaction with SMC5. AtMMS21 acts in DSB amelioration and stem cell niche maintenance during root development [24]. Hence, AtMMS21 is involved in cell division, differentiation, expansion and survival during plant development. The highly coordinated processes of cell division, differentiation, and expansion that take place during gametophyte development require precise fine-tuning of gene regulatory networks [25]. However, whether and how AtMMS21 participates in regulating the gametophyte development and reproductive processes remains unclear.

In the present study, we provide cell-biological and molecular evidence that AtMMS21 is required for fertility in Arabidopsis. Mutations in AtMMS21 cause semi-sterility with aberrant gamete, indicating that the gene is essential for gametogenesis. Furthermore, mms21-1 mutant cells exhibit chromosome fragmentation and mis-segregation during meiosis. Transcription level for several meiotic genes are also altered in mms21-1 buds. These observations suggest that AtMMS21 plays an important role in meiosis and gametophyte development.

Results

mms21-1mutant shows severely reduced fertility

Previous studies showed that mutation of AtMMS21/HPY2 resulted in severe developmental defects [21, 22, 24]. To determine whether AtMMS21 regulates the reproductive development, we first analyzed the fertility of mms21-1 and wild-type plants. In their reproductive phase, mms21-1 plants were bushy with short siliques (Figure 1A-D). Mean silique length was reduced to 6.3 ± 0.44 mm in mms21-1, compared with 14.1 ± 0.18 mm in the wild-type (Figure 1I). Ten days after pollination, dissected siliques from mms21-1 plants showed severely reduced seed-set and unfertilized ovules (Figure 1H). Later in mature siliques, the mean seed-set was only 13.7 ± 1.33 per silique, accounting for 22.2% of the normal seed-set in the wild-type (Figure 1J). Some of the mutant seeds were abnormal in appearance with a dark and shrunken seed coat (Figure 1F). The percentage of aborted seeds in mms21-1 is approximatly 48.3%, while only 0.4% in wide-type (Figure 1K). Furthermore, we analyzed fertility in the transgenic plants expressing 35S::AtMMS21-GFP in mms21-1, and found that the expression of AtMMS21-GFP could rescue the semisterile phenotype of mms21-1 (Figure 1C,D,G,H), indicating that the impaired fertility of the mms21-1 is caused by the absence of AtMMS21. Therefore, these results suggested that AtMMS21 is essential to fertility in Arabidopsis.

Figure 1
figure 1

mms21-1 plants exhibit reduced fertility. (A-C) Morphology of 6-week-old wild-type, mms21-1 and 35:MMS21 mms21-1 plants under long-day growth conditions. (D) Primary inflorescences of wild-type, mms21-1 and 35:MMS21 mms21-1 plants. (E-G) Seed phenotype in wild-type and mms21-1 plants. (H) Dissected silique form wild-type , mms21-1 and 35:MMS21 mms21-1 plants. mms21-1 showing severly reduced seed-set and unfertilized ovules. (I) The lengths of siliques in wild-type , mms21-1 and 35:MMS21 mms21-1. (J) Numbers of seeds per silique in wild-type, mms21-1 and 35:MMS21 mms21-1. (K) Percentage aborted seeds per silique in wild-type , mms21-1 and 35:MMS21 mms21-1. Bars = 5 cm in (A-C), 1 cm in (E), 5 mm in (H).

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Decreased fertility in mms21-1is caused by both abnormal male and female fertility

To answer the question of whether male or female fertility was affected by the mutation, we performed reciprocal cross-pollinations between mms21-1 and wild-type plants. In reciprocal cross-pollinations, wild-type pollen showed active pollen tube growth to the base of the wild-type pistil in 12 h, and the average size of mature siliques and number of seeds per silique from this cross were equivalent to those of self-pollinated wild-type plants (Figure 2A, F, G). By contrast, mms21-1 pollen did not show normal fertilization in either mms21-1 or wild-type pistils. Unfertilized ovules were random distributed in the mature siliques and a high percentage of shrunken seeds (Figure 2B, D, G), and short siliques and small numbers of seeds per silique, (Figure 2E, F), indicating that the function of the pollen was compromised in the mms21-1 mutants.

Figure 2
figure 2

In vivo reciprocal cross-pollination of mms21-1 to wild-type plants. (A-D) Pistils were fixed at 12 h after pollination, and pollen tube growth was examined by aniline blue staining, bars = 500 μm. The remaining pollinated pistils ripened into mature siliques in 14 d, and siliques were then dissected for examination of fertilized ovules. Arrows indicate the pollen tube front in the pistil. Asterisks designate unfertilized ovules in the silique. (E-H) In another set of reciprocal cross-pollinations, sizes of mature siliques, numbers of seeds per silique and percentage aborted seeds per silique were examined 14 d after pollination. Control flowers were allowed to self-pollinate (asterisks). Data are shown as mean ± SD. (E) Percentage of pollen tubes growing to the base of the pistil. Bars = 5 mm.

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Cross-pollination of wild-type pollen onto mms21-1 pistils resulted in better fertilization and silique sizes (Figure 2C). However, the siliques size and seed number per silique were still decreased in this cross, compared with the wild-type self-cross (Figure 2E, F). Cross-pollination of mms21-1 pollen onto wild-type pistil showed a lower percentage in pollen tube growth to the base of the pistil (Figure 2H). Taken together, our reciprocal cross-pollination studies suggested that AtMMS21 has a function in both male and female fertility.

mms21-1mutant shows reduced pollen number, viability, germination and abnormal pollen tube growth

To further characterize the semisterile phenotype of mms21-1 plant, we first examined the effects of the mms21-1 mutation on male fertility. Unlike wild-type (Figure 3A), there were few pollen grains produced on the surfaces of anthers and stigma in mms21-1 flowers (Figure 3B). 861 ± 135(n = 90) pollen grains per wild-type flower but only about 136 ± 53(n = 90) pollen grains were observed in mms21-1flowers. To assess pollen grain viability, anthers and mature pollen grains from both the wild-type and mutant flowers were stained with Alexander’s solution [26]. Wild-type mature anthers were in uniform size, and the pollen grains stained red, which indicates viability (Figure 3C, E). In contrast, mms21-1 mature anthers were variable in size and shape (Figure 3D, F). Pollen grains in mms21-1 plants were generally bigger with about 30.0% nonviable pollen grains, as indicated by blue staining, whereas the wild-type produced <1.9% abnormal pollen (Figure 3F). In vitro pollen germination and pollen tube growth assays were also performed. In mms21-1, pollen tube initiation and growth occurred in only 31.3% of the pollen grains while 78.2% of wild-type pollen germinated (Figure 3 G-I). Particularly, mms21-1 pollen tubes showed a variable phenotypes and abnormal morphologies. For example, long pollen tubes exhibiting branched tips (red arrows in Figure 3I) and short swollen pollen tubes were observed (white arrows in Figure 3I). These results indicated that AtMMS21 mutation affects pollen number, viability, germination and tube growth.

Figure 3
figure 3

Mutation in AtMMS21 affects pollen number, viability, germination and growth. (A-B) Flower of wild-type and mms21-1 plants. mms21-1 produced dramatically reduced pollen grains. (C-D) Anthers of wild-type and mms21-1 plants, which are stained with Alexander’s solution. (E-F) Pollen grains of wild-type and mms21-1 plants, which are stained with Alexander’s solution. The red-stained cytoplasm indicates viable pollen grains, whereas nonviable pollen grains are stained blue. (G) Percentage of pollen germination. (G-I) In vitro pollen tube growth assay. White arrows indicate long pollen tubes exhibiting branched tips, and red arrows indicate short pollen tubes tip with obviously swollen. Bars = 5 mm in (A-B), 2 mm in (E-F, H-I).

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Mutation of AtMMS21causes defects in gametogenesis

To determine which step of pollen development is affected by the mms21-1 mutation, paraffin-cross sections of anthers stained with toluidine blue from various developmental stages were analyzed (Additional file 1: Figure S1). In Arabidopsis, anther development can be divided into 14 well-ordered stages by morphological characteristics [27]. In mms21-1, the early 6 stages of pollen development appeared normal compared with wild-type (Figure 4A, F; Additional file 1: Figure S1). Alteration were first observed at tetrad stage, wild-type meioses produced four uniform spores, while the mutant produced a mixture of dyads, triads, and tetrads (Figure 4B, G, K, O; Additional file 2: Figure S2). At stage 8, microspores are typically released from the tetrads. Microspores were angular in shape in wild-type plants, whereas microspores of mms21-1 plants appeared of various sizes (Figure 4C, H). During stage 11, wild-type microspores underwent asymmetric mitotic divisions and generated a significant pollen wall (Figure 4D). In contrast, in mms21-1, most of the microspores were degenerated (Figure 4I). Eventually, pollen grains of the wild-type were released when anther dehiscenced, whereas most of the mutant microspores were degenerated, leaving an empty locule (Figure 4E-J).

Figure 4
figure 4

Male gametophyte development is defective in mms21-1 mutants. Anther development at stages 6, 7, 8, 11, 13 in the wild-type (A–E) and mms21-1 mutant (F–J). (A, F) Anther stage 6. PMCs have separated and are ready to undergo meiosis. (B, G) Anther stage 7. Uniform size tetrads (Tds) in wild-type, dyads and triads in mms21-1 mutant. (C, H) Anther stage 8. Note the dyads in mms21-1 mutant. (D, I) Anther stage 11. Pollen were uniform size in wild-type but variable size and shrunken in mms21-1 mutant. (E, J) Anther stage13. In mms21-1 mutant pollen grains were in variable size and shrunken.MC, meiotic cell; Tds, tetrads; MSp, microspores; PG, pollen grains; T, tapetum. Bars = 20 μm. (K-N) DAPI staining of wild-type anthers at different development stages. Pollen grains are of uniform size in the wild-type. (O-R) DAPI staining of mms21-1 anthers at stages equivalent to (K-N). Arrows in (O) indicate that there are dyads and triads at tetrad stage in mms21-1. Arrows in (P) indicate that visible variable size (red arrow) and some had two nuclei (white arrow) in uninucleus microspore stage in mms21-1. Arrows Q indicate that abnormal nuclear contents pollen Arrows in R indicate larger pollen (white arrow) and pollen with no DAPI staining (red arrow) in mms21-1. Bars = 20 μm. S, AtMMS21 expression was detected in PMCs by in situ hybridization. T, No hybridization signal was detected when a sense probe was used.

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To more precisely define the developmental defect in mms21-1 pollen, developing mms21-1 pollen were stained with DAPI and observed at different developmental stages. The mms21-1 meiotic products were a mixture of dyads, triads and tetrads, while the WT tetrads had four equal-sized spores enclosed in a callose wall (Figure 4K, O). At the uninucleate stage, some abnormal microspores in mms21-1 exhibited no fluorescence or contained two nuclei within one exine wall (Figure 4P). In the bicellular pollen stage, some of the mms21-1 pollen became shrunken and lacked DAPI staining compared with wild-type (Figure 4M, Q). The size difference became more pronounced at the tricellular stage, as normal pollen continued to approach maturity, while mutants were losing nuclear content and becoming distorted (Figure 4R). We also examined expression patten of AtMMS21 by in situ hybridization. Transverse sections of anthers showed strong hybridization signals in pollen mother cells (PMCs; Figure 4S); no signals were detected when a sense probe was used (Figure 4T). Loss of AtMMS21 function also causes defects in female gametogenesis. Normally the diploid megaspore mother cell undergoes meiosis and gives rise to four haploid nuclei. Subsequently, three megaspores undergo cell death, with the remaining, functional megaspore proceeding into megagametogenesis. Examination of cleared ovules in mms21-1 mutant plants showed that some megaspore mother cells appeared to abort either before or during meiosis , giving rise to embryo sacs containing one to five nuclei (Additional file 3: Figure S3). These data indicated that AtMMS21 is important for gametogenesis, both during male and female gametophyte development.

Disruption of AtMMS21leads to defects in chromosome behavior during meiosis

The defects in gametophyte development described above could arise from defective meiosis. Therefore we examined chromosome spreads from various stages of meiosis of wild-type and mms21-1 anthers. In wild-type meiosis, chromosomes were clearly single and unpaired in leptotene (Figure 5A) and underwent synapsis between homologous chromosomes at the zygotene stage (Figure 5B) until its completion in pachytene (Figure 5C). At diakinesis stage, homologous chromosomes desynapsed and then underwent further condensation to form five bivalents (Figure 5D). The five bivalents aligned at the division plane at metaphase I (Figure 5E). At anaphase I homologous chromosomes separated from each other and moved to opposite poles (Figure 5F). Subsequently tetrads formed at anaphase II (Figure 5G). In mm21-1mutant plants, the early development stages of meiotic nuclei, in other words from leptotene to pachytene, appeared to proceed normally (Figure 5H-J). Alterations were observed at diakinesis, in diakinesis, chromosomes were further condensed, but in mms21-1 it appeared to be less condensed than wild type (Figure 5K). Interestingly, we did not observe distinct metaphase I in the mms21–1. Furthermore, chromosome fragments and bridges between bivalents were observed in anaphase I (Figure 5L-N) In anaphase II, segregation of the sister chromotids were also disturbed leading to irregular meiotic products with variable DNA contents (Figure 5O-Q). As some lagging chromosome dispersed throughout the cytoplasm, typical tetrads were rarely found (Figure 5R-S). These observations showed that loss function of AtMMS21 cause abnormal meiotic chromosome behavior.

Figure 5
figure 5

The mms21-1 plants exhibit defects during meiosis. DAPI staining of meiosis stages from wild-type pollen mother cells. Various stages of meiotic chromosome spreads from wild-type (A–G) and mms21–1 (H-S) are illustrated (A, H) Leptotene stage. (B, I) Zygotene stage. (C, J) Pachytene stage. (D, K) Diakinesis stage. (E) Metaphase I. (F, L-N) Anaphase I. (G, O-S) Telophase II. Arrows indicate abnormal chromosomal fragments (I, M, O-R), chromosome bridges (N), and lagging chromosome (S). Bars = 10 μm.

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mms21-1mutants exhibits altered transcript levels for meiotic genes

Meiocytes exhibit abnormal chromosome behavior, we investigated whether loss of AtMMS21 affected meiotic gene expression, which includes ASY1 [28], ZYP1 [29], DMC1 [30], TAM [31], MSH4 [32], PHS1 [33], RAD51 [34], RAD51C [35], RBR1 [36], SPO11-1 [37], and SPO11-2 [38] by the qRT-PCR. Although transcript levels for DMC1, MSH4, SPO11-2 and TAM1 were similar in the buds of wild-type (Figure 6A), many meiotic gene expression were changed in the flower buds of mms21-1. For example, transcripts for SPO11-1 and RAD51 were found to be increased approximately 3 and 3.5 fold, respectively. RBR is essential for inter-homologue recombination and synapsis [36], and transcripts for RBR were up-regulated in mms21-1 mutant. ZYP1 encodes a synaptonemal complex protein and ASY1 encods an axis-associated protein in Arabidopsis [28, 29]; ZYP1a and ASY1 transcript levels were down-regulated compared with wild-type plants. In addition, transcripts of cohesin, condensin, SMC5/6 complex and SMC-like genes in mms21-1 plants were elevated, with the exception of SYN1 and SWI1-like (Figure 6B). Particularly, the transcript abundance level of SWI1 was dramatically increased.

Figure 6
figure 6

Expression analyses of meiotic and structural maintenance of chromosome genes in wild-type and mms21-1 flowers. Quantitative PCR was used to measure transcript levels of meiotic genes in buds RNA isolated from wild-type and mms21-1 mutant plants. (A) Expression analysis of select synapsis, recombination in wild-type and mms21-1 plants. (B) Expression analysis of select cohesin, condensin, SMC5/6 complex and SMC-like genes in wild-type and mms21-1 plants. Data presented here represent three biological replicate experiments. Each quantitative RT-PCR was repeated at least three times on each biological replicate. Bars are averages ± SE.

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mms21-1mutant exhibits reduced SUMOylation levels in pollen grains

Because AtMMS21 encodes a SUMO E3 ligase and mutation of AtMMS21 causes defective gametocyte, it is reasonable to assume that accumulation of SUMO-protein conjugates in generative cell were altered in mms21-1 mutant. An immunoblot of total pollen grain proteins by anti-AtSUMO1 demonstrated a difference in the SUMO conjugates in wild-type and mms21-1, as some SUMOylation conjugates were missing in mms21-1 pollen grains proteins (Figure 7). These results suggested that AtMMS21 is involved in the SUMOylation of Arabidopsis pollen grains proteins, and may regulate the gametophyte developmental process through the SUMOylation pathway.

Figure 7
figure 7

SUMOylation profiles of pollen proteins in wild-type and mms21-1 plants. Pollen protein extracts were analyzed by protein gel blots using anti-SUMO1polyclonal antibodies to detect SUMO-protein of wild-type and mms21-1. Arrows indicate missing SUMO conjugates in mms21-1. Coomassie brilliant blue staining of total protein was used as the loading control.

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Discussion

SUMOylation and the components of the SUMO conjugation machinery are essential for viability, as shown by the embryo lethality of the mutations in SAE2 or SCE or both in SUMO1 and SUMO2 [39]. However, the role of SUMOylation for gametophyte development is poorly understood because of the zygotic lethality. Here, we used a viable mutant without functional SUMO E3 ligase AtMMS21 for studying the function of SUMOylation in reproductive development. First, we examined T-DNA mutants of AtMMS21 [21], focusing on silique size and seed set, which are direct indicators of successful fertility. The mms21-1 mutant exhibits a drastic reduction in fertility, embodied by a shorter silique length with a smaller seed set than those in the wild-type (Figure 1). Examination of the male fertility indicated that mutant anthers produce fewer functional pollen grains (Figure 3). Cytological observation of various developmental stages in mms21-1 pollen revealed that the products of meiosis in the mutant were mostly dyads of spores instead of tetrads (Figure 4). Similarly, mutant meiosis produced abnormal embryo sacs during female gametogenesis (Figure 2). These results are consistent with our reciprocal cross study suggesting that AtMMS21 has crucial roles in both male and female gametogenesis.

Gametogenesis is an essential process for plant reproduction and the roles of the ubiquitin system in different processes during gametogenesis have been studied extensively [1]. However, although ubiquitin-like proteins SUMO have emerged as a key regulator of plant development and stress response [40], there are currently little data supporting a specific role of the SUMO system in the control of reproduction. Our data presented a regulatory framework for the action of AtMMS21-dependent SUMOylation in reproductive development. The loss of function mutant mms21-1 is still viable probably due to the action of another SUMO E3 ligase SIZ1 [41]. The siz1-2 and mms21/hpy2 double mutant results in embryonic lethality, supporting the notion that AtMMS21 and SIZ1 have overlapping functions [42]. Mature female gametophytes were rapidly disrupted in the absence of the SIZ1 protein, while pollen developed well, indicating that SIZ1 plays important roles in sustaining the stability of the mature female gametophyte [42]. However, unlike the SIZ1, AtMMS21 is involved in the development and the female and male gametophyte. Interestingly, AtMMS21 was recently shown to be expressed highly in reproductive organs such as anther using a GUS reporter construct [42]. The expression data supported the role of AtMMS21 during gametophyte development inferred from the mms21-1 mutant lines. The severe defects in mms21-1 gametophyte indicated that AtMMS21 is vital for fundamental processes (e.g. meiosis), thereby ensuring normal reproductive development in Arabidopsis.

The precise transmission of chromosomes from mother to daughter cells is a highly controlled process that requires members of the SMC (structural maintenance of chromosome) protein family. Although cohesin (SMC1/3) and condensin (SMC2/4) complexes have been reported to be involved in many aspects of meiosis, the role of the SMC5/6 complex in meiosis remains elusive. Here, we demonstrated that a SMC5/6 subunit, AtMMS21, is essential during Arabidopsis gametogenesis. The lack of AtMMS21 resulted in severely disrupted meiosis with bridges between chromosomes and chromosome fragmentations during meiosis I, leading to the unequal distribution of meiotic products and polyad formation during meiosis II (Figure 5). AtMMS21 is an important regulator of cell cycle progression [23, 24]. It is possible that the dyad cells may result from delayed progression of meiosis. During meiosis, a germ cell will purposely create double-strand breaks which is induced by the protein SPO11 to promote chiasmata formation, and the programmed DSBs which are repaired by HR [43]. If the DSBs remain unrepaired while the cell cycle continues, it will possibly lead to fragmentation and mis-segregation [44]. Recent study demonstrated that AtMMS21, a SMC5/6 complex sub-unit, is involved in DSB repair [26]. Chromosome fragmentation and mis-segregation observed in meiosis of mms21-1 may due to defective DSB repair. The chromosome fragmentation may result from failure of DSB repair or non-condensed entangled chromosomes pulled to break by the spindle. This notion is consistent with previous finding that the yeast SMC5/6 complex is required to ensure proper chromosome behavior during meiosis [45, 46]. In addition, several genes associated with DSB accumulation or formation, such as RAD51 RAD51C and SPO11-1 were increased in mms21-1 flower buds (Figure 6A), suggesting that mms21-1 generative cells may contain unrepaired DSBs. Therefore, it will be interesting to examine whether AtMMS21 is recruited to mitotic DSBs and function in meiotic recombination during meiosis. Although the meiotic functions of AtMMS21 need to be investigated, our data demonstrated that AtMMS21 is required for proper chromosome behavior and successful meiotic divisions.

Meiotic roles have been discovered for cohesins and condensins in plants [10]. Here we show that a SMC5/6 associated protein AtMMS21 is important for meiosis. Furthermore, several genes encode various components of the different SMC complexes in mms21-1 plants display increased transcription level (Figure 7B). SMC complexes and their associated proteins are essential for sister chromatid cohesion, chromosome condensation, DNA repair and recombination. It is tempting to speculate that AtMMS21 gene may affect meiotic process indirectly by altering the expression of SMC complexes and their associated genes. When exposured to DNA damaging agents all subunits of cohesin become SUMOylated, such as the SUMOylation of SCC1 is carried out by the SUMO E3 ligase MMS21 in yeast [47]. Therefore, further analysis of the protein expression of SMC complexes in the mms21-1 meiocytes and the relation between AtMMS21 and cohesin/condensin complexes will help to clarify the precise function of AtMMS21 during meiosis. At the protein level, our results demonstrated that SUMOylation level in mms21-1 pollen grains proteins is different from the wild-type (Figure 7), indicating that AtMMS21-mediated SUMOylation may participate in generative cell formation. Identification and functional analyses of sumoylated proteins related to AtMMS21 are necessary for the understanding how AtMMS21-dependent SUMOylation participates in meiosis and gametophyte development.

Conclusions

In conclusion, our studies found that Arabidopsis MMS21 is important for gametogenesis, both during male and female gametophyte development. The loss of the Arabidopsis MMS21 causes reduced pollen number, viability, germination and abnormal meiotic chromosome behavior. Several transcripts for meiotic genes related to chromosome maintenance and behavior are altered in the mms21-1 plant. Futhermore, SUMO-protein conjugates in the mms21-1 pollen grains are different from those in wild-type. Thus, these results indicated that AtMMS21 mediated SUMOylation may stabilize the expression and accumulation of meiotic proteins in the gametophyte development.

Methods

Plant materials and growth conditions

The mms21-1 mutant and 35S:AtMMS21 Arabidopsis (Arabidopsis thaliana; Columbia-0 ecotype) were isolated as described previously [21]. Arabidopsis plants were grown in a controlled growth room at 22 ± 2°C under long-day conditions (16-h light/8-h dark). For in vitro experiments, seeds were surface-sterilized for 2 min in 75% ethanol, followed by 5 min in 1% NaClO solution and washed five times in sterile distilled water, plated on growth medium (MS medium, 1.5% sucrose and 0.8% agar), vernalized at 4°C for 2 days in the dark and then exposed to white light.

In vitro pollen tube growth assay

Plants were removed from the growth chamber for 2 h before pollen was removed from flowers. Pollen was grown on solid germination medium (0.01% boric acid, 5 mM CaCl2, 5 mM KCl,1 mM MgSO4, 10% sucrose pH 7.5, 1.5% low-melting agarose) [48] at room temperature in dark. Pollen tube length and tip morphology were examined at various time points (2 to 16 h) using a Leica dissecting microscope for higher magnification. The relative length of pollen tubes was measured at 12 h using the tool DIGIMIZER 3.2.1.0.

Study of in vivo pollen tube growth and seed formation in siliques

To examine in vivo pollen tube growth, about 10 mature flowers at stage 14 [49] were fixed in acetic acid/ethanol (1:3) solution. Fixed floral tissues were cleared in 4 M NaOH and stained with aniline blue following a previously published method [50]. Pollen tube growth in the pistil was examined using a fluorescent compound microscope (Leica microscope DM2500).

To evaluate fertilization, mature siliques were measured for their lengths and dissected to identify aborted seeds. Siliques were also decolorized by incubation in 100% ethanol at 37°C overnight to visualize the seed set.

For in vivo reciprocal cross-pollination, 40 floral buds at stage 12 [49] were emasculated per cross a day before hand-pollination. Fresh pollen at flower stage 13 [49] was fully applied to the stigma of the emasculated flower. After a 12-h pollination, the pollinated pistil was fixed with 25% acetic acid in ethanol, hydrated with an ethanol series (70%, 50% and 30% ethanol), and treated with 8 M NaOH overnight to allow softening, the pollen tube distribution in each silique was observed after staining with 0.1% (w/v) aniline blue solution containing 108 mM K3PO4 at pH 11 and 2% glycerol, and examined as described above.

For the fertilization study, half of the pollinated flowers were further grown in the growth chamber for 8 to 10 d. Siliques were dissected or decolorized at maturity to examine seed set.

Microscopic investigations of anther development after paraffin-section

Anthers were fixed in FAA (10% formaldehyde, 3% acetic acid and 43.5% ethanol) placed under vacuum for 1 h and then keep room temperature. After dehydration in a graded ethanol series and diaphaneity in clearing medium, the material was embedded in Paraffins (from HuaShenPai). Sections (6 μm) were obtained with a Leica Reichert Supernova microtome, placed on glass slides, and stained with a solution of 1%(w/v) toluidine blue O (toluidine blue O 1 g, 95% alcohol 4 mL, 10% acetic acid 10 mL, distilled water 86 mL). Sections examined using a Leica fluorescent compound microscope and Images were captured with a Leica DFC420 camera, and processed with Leica Application Suite software.

The images of whole morphology of WT and mutant were captured using SONY DSC-H50 camera. And the flowers, siliques and seeds morphology were examined using a Leica dissecting microscope.

Pollen grain viability assay

Anthers were removed from flowers and mounted in a drop of Alexander’s [26] stain under a cover glass to study the abundance of pollen grains, and mature pollen grains from the WT and mutant flower buds and stained with Alexander’s staining solution, and examined using a fluorescent compound microscope (Leica microscope).

Light and fluorescence microscopy

The DAPI (4,6-diamino-2-phenylindole dihydrochloride) staining of chromosomes in the male meiocytes was performed according to the method reported by Ross et al. [51]. The young buds were fixed with Carnoy’s fixative. Fixed buds were rinsed in five changes of 1 min washes in acetic buffer (10 mM sodium acetic, pH 4.5). Buds were digested with 0.3% cytohelicase, 0.3% cellulose and 0.3% pectolyase in distilled water for an hour at 37°C, and then washed with 10 mM acetic buffer two times. A drop of 60% acetic acid was added to the glass slide and the slide was incubated at 42°C for 1–2 min. Slides were stained with 2 mg/mL of DAPI (Sigma-Aldrich) and examined by a Leica DM2500 fluorescence microscope.

For the analysis of spores at earlier stages, single anthers were dissected from isolated buds using a dissecting microscope (Zeiss, Stemi SV8).Anthers were disrupted on microscope slides using dissecting needles and gently squashed in DAPI staining solution (0.8 μg/mL) under a coverslip.

To determine at which developmental stage the mutant defection, inflorescences containing buds at different developmental stages were fixed in ethanol: acetic acid (3:1; v/v) and stored at 4°C. Buds were dissected on a microscope slide and microspores or pollen were stained in 0.8 μg/mL DAPI.

Differential interference contrast microscopy was used to observe female gametophytes that had been fixed in ethanol: acetic acid (3:1) and cleared using chloral hydrate solution (8 g of chloral hydrate, 1 mL of glycerol, and 2 mL of water). Images were captured with Leica DM2500 microscope.

Expression analysis

Total RNA was extracted from buds with the TRizol (Invitrogen), and 10 μg of RNA was treated with DNase I (TAKARA, http://www.takara-bio.com) and used for cDNA synthesis with an oligo (dt) primer and a First Strand cDNA Synthesis Kit (TAKARA). PCR was performed with the SYBR-Green PCR Mastermix (TAKARA) and amplification was monitored on a MJR Opticon Continuous Fluorescence Detection System (Bio-Rad). At least three biological replicates were performed, with three technical replicates for each sample. The sequences of primers used in these studies are presented in Additional file 4: Table S1.

The DIG RNA Labeling Kit (Roche) and DIG ProbeSynthesis Kit (Roche) were used for the in situ hybridization. An AtMMS21-specific cDNA fragment of 297 bp was amplified and cloned into the pSKvector. Antisense and sense digoxigenin-labeled probes were prepared as described by Zhu et al. [52]. The primers for the in situ hybridization were hmms21F (5′—GGATCC ATTCTGTGGCTGAGTTATTG-3′) and mms21R (5′-CTGCAG ATGTCATGTTTAGAAGAGGG-3′).

Analysis of SUMOylation profiles

Total protein of mature pollen grains were extracted with extraction buffer (50 mM PBS PH = 7.4, 200 mM NaCl, 10 mM MgCl2, Glycerol 10%, add protease inhibitor cocktail tablets 10 mL/one mini tablet, Roche) and separated by SDS–PAGE. Proteins were separated on a 10% polyacrylamide gel and transferred to PVDF membrane. Polyclonal SUMO1 antibody (Agrisera) diluted in the ratio 1:3000 was applied followed by an anti-rabbit IgG coupled to HRP and detected using ECL plus (Amersham Pharmacia).

References

  1. Ma H, Sundaresan V: Development of flowering plant gametophytes. Curr Top Dev Biol. 2010, 91: 379-412.

    Article  CAS  PubMed  Google Scholar 

  2. Yang WC, Shi DQ, Chen YH: Female gametophyte development in flowering plants. Annu Rev Plant Biol. 2010, 61: 89-108. 10.1146/annurev-arplant-042809-112203.

    Article  CAS  PubMed  Google Scholar 

  3. Borg M, Brownfield L, Twell D: Male gametophyte development: a molecular perspective. J Exp Bot. 2009, 60: 1465-1478. 10.1093/jxb/ern355.

    Article  CAS  PubMed  Google Scholar 

  4. Alvarez-Buylla ER, Benitez M, Corvera-Poire A, Chaos Cador A, de Folter S, de Buen AG, Garay-Arroyo A, Garcia-Ponce B, Jaimes-Miranda F, Perez-Ruiz RV, Pineyro-Nelson A, Sanchez-Corrales YE: Flower development. Arabidopsis Book. 2010, 8: e0127-

    Article  PubMed  PubMed Central  Google Scholar 

  5. Gallois JL, Guyon-Debast A, Lecureuil A, Vezon D, Carpentier V, Bonhomme S, Guerche P: The Arabidopsis proteasome RPT5 subunits are essential for gametophyte development and show accession-dependent redundancy. Plant Cell. 2009, 21: 442-459. 10.1105/tpc.108.062372.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Harrison CJ, Alvey E, Henderson IR: Meiosis in flowering plants and other green organisms. J Exp Bot. 2010, 61: 2863-2875. 10.1093/jxb/erq191.

    Article  CAS  PubMed  Google Scholar 

  7. Jones GH, Armstrong SJ, Caryl AP, Franklin FC: Meiotic chromosome synapsis and recombination in Arabidopsis thaliana; an integration of cytological and molecular approaches. Chromosome Res. 2003, 11: 205-215. 10.1023/A:1022831724990.

    Article  CAS  PubMed  Google Scholar 

  8. Murnane JP: Telomeres and chromosome instability. DNA Repair (Amst). 2006, 5: 1082-1092. 10.1016/j.dnarep.2006.05.030.

    Article  CAS  Google Scholar 

  9. Liu Z, Makaroff CA: Arabidopsis separase AESP is essential for embryo development and the release of cohesin during meiosis. Plant Cell. 2006, 18: 1213-1225. 10.1105/tpc.105.036913.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Schubert V: SMC proteins and their multiple functions in higher plants. Cytogenet Genome Res. 2009, 124: 202-214. 10.1159/000218126.

    Article  CAS  PubMed  Google Scholar 

  11. Yuan L, Yang X, Ellis JL, Fisher NM, Makaroff CA: The Arabidopsis SYN3 cohesin protein is important for early meiotic events. Plant J. 2012, 71: 147-160. 10.1111/j.1365-313X.2012.04979.x.

    Article  CAS  PubMed  Google Scholar 

  12. Jiang L, Xia M, Strittmatter LI, Makaroff CA: The Arabidopsis cohesin protein SYN3 localizes to the nucleolus and is essential for gametogenesis. Plant J. 2007, 50: 1020-1034. 10.1111/j.1365-313X.2007.03106.x.

    Article  CAS  PubMed  Google Scholar 

  13. Cai X, Dong F, Edelmann RE, Makaroff CA: The Arabidopsis SYN1 cohesin protein is required for sister chromatid arm cohesion and homologous chromosome pairing. J Cell Sci. 2003, 116: 2999-3007. 10.1242/jcs.00601.

    Article  CAS  PubMed  Google Scholar 

  14. Lam WS, Yang X, Makaroff CA: Characterization of Arabidopsis thaliana SMC1 and SMC3: evidence that AtSMC3 may function beyond chromosome cohesion. J Cell Sci. 2005, 118: 3037-3048. 10.1242/jcs.02443.

    Article  CAS  PubMed  Google Scholar 

  15. Chelysheva L, Diallo S, Vezon D, Gendrot G, Vrielynck N, Belcram K, Rocques N, Marquez-Lema A, Bhatt AM, Horlow C, Mercier R, Mezard C, Grelon M: AtREC8 and AtSCC3 are essential to the monopolar orientation of the kinetochores during meiosis. J Cell Sci. 2005, 118: 4621-4632. 10.1242/jcs.02583.

    Article  CAS  PubMed  Google Scholar 

  16. Siddiqui NU, Rusyniak S, Hasenkampf CA, Riggs CD: Disruption of the Arabidopsis SMC4 gene, AtCAP-C, compromises gametogenesis and embryogenesis. Planta. 2006, 223: 990-997. 10.1007/s00425-006-0234-z.

    Article  CAS  PubMed  Google Scholar 

  17. Siddiqui NU, Stronghill PE, Dengler RE, Hasenkampf CA, Riggs CD: Mutations in Arabidopsis condensin genes disrupt embryogenesis, meristem organization and segregation of homologous chromosomes during meiosis. Development. 2003, 130: 3283-3295. 10.1242/dev.00542.

    Article  CAS  PubMed  Google Scholar 

  18. Liu Cm CM, McElver J, Tzafrir I, Joosen R, Wittich P, Patton D, Van Lammeren AA, Meinke D: Condensin and cohesin knockouts in Arabidopsis exhibit a titan seed phenotype. Plant J. 2002, 29: 405-415. 10.1046/j.1365-313x.2002.01224.x.

    Article  PubMed  Google Scholar 

  19. Watanabe K, Pacher M, Dukowic S, Schubert V, Puchta H, Schubert I: The structural maintenance of chromosomes 5/6 complex promotes sister chromatid alignment and homologous recombination after DNA damage in Arabidopsis thaliana. Plant Cell. 2009, 21: 2688-2699. 10.1105/tpc.108.060525.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  20. Tzafrir I, Pena-Muralla R, Dickerman A, Berg M, Rogers R, Hutchens S, Sweeney TC, McElver J, Aux G, Patton D, Patton D, Meinke D: Identification of genes required for embryo development in Arabidopsis. Plant Physiol. 2004, 135: 1206-1220. 10.1104/pp.104.045179.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  21. Huang L, Yang S, Zhang S, Liu M, Lai J, Qi Y, Shi S, Wang J, Wang Y, Xie Q, Yang C: The Arabidopsis SUMO E3 ligase AtMMS21, a homologue of NSE2/MMS21, regulates cell proliferation in the root. Plant J. 2009, 60: 666-678. 10.1111/j.1365-313X.2009.03992.x.

    Article  CAS  PubMed  Google Scholar 

  22. Ishida T, Fujiwara S, Miura K, Stacey N, Yoshimura M, Schneider K, Adachi S, Minamisawa K, Umeda M, Sugimoto K: SUMO E3 ligase HIGH PLOIDY2 regulates endocycle onset and meristem maintenance in Arabidopsis. Plant Cell. 2009, 21: 2284-2297. 10.1105/tpc.109.068072.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  23. Zhang S, Qi Y, Yang C: Arabidopsis SUMO E3 ligase AtMMS21 regulates root meristem development. Plant Signal Behav. 2010, 5: 53-55. 10.4161/psb.5.1.10158.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  24. Xu P, Yuan D, Liu M, Li C, Liu Y, Zhang S, Yao N, Yang C: AtMMS21, an SMC5/6 complex subunit, is involved in stem cell niche maintenance and DNA damage responses in arabidopsis roots. Plant Physiol. 2013, 161: 1755-1768. 10.1104/pp.112.208942.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  25. Berr A, McCallum EJ, Ménard R, Meyer D, Fuchs J, Dong A, Shen W-H: Arabidopsis set domain group2 is required for H3K4 trimethylation and is crucial for both sporophyte and gametophyte development. Plant Cell. 2010, 22: 3232-10.1105/tpc.110.079962.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  26. Alexander MP: Differential staining of aborted and nonaborted pollen. Stain Technol. 1969, 44: 117-122.

    Article  CAS  PubMed  Google Scholar 

  27. Sanders PM, Bui AQ, Weterings K, McIntire KN, Hsu YC, Lee PY, Truong MT, Beals TP, Goldberg RB: Anther developmental defects in Arabidopsis thaliana male-sterile mutants. Sex Plant Reprod. 1999, 11: 297-322. 10.1007/s004970050158.

    Article  CAS  Google Scholar 

  28. Armstrong SJ, Caryl AP, Jones GH, Franklin FCH: Asy1, a protein required for melotic chromosome synapsis, localizes to axis-associated chromatin in Arabidopsis and Brassica. J Cell Sci. 2002, 115: 3645-3655. 10.1242/jcs.00048.

    Article  CAS  PubMed  Google Scholar 

  29. Higgins JD, Sanchez-Moran E, Armstrong SJ, Jones GH, Franklin FC: The Arabidopsis synaptonemal complex protein ZYP1 is required for chromosome synapsis and normal fidelity of crossing over. Genes Dev. 2005, 19: 2488-2500. 10.1101/gad.354705.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  30. Klimyuk VI, Jones JDG: AtDMC1, the Arabidopsis homologue of the yeast DMC1 gene: characterization, transposon-induced allelic variation and meiosis-associated expression. Plant J. 1997, 11: 1-14. 10.1046/j.1365-313X.1997.11010001.x.

    Article  CAS  PubMed  Google Scholar 

  31. Wang Y: Progression through Meiosis I and Meiosis II in arabidopsis anthers is regulated by an a-type cyclin predominately expressed in prophase I. Plant Physiol. 2004, 136: 4127-4135. 10.1104/pp.104.051201.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  32. Higgins JD, Armstrong SJ, Franklin FCH, Jones GH: The Arabidopsis MutS homolog AtMSH4 functions at an early step in recombination: evidence for two classes of recombination in Arabidopsis. Genes Dev. 2004, 18: 2557-2570. 10.1101/gad.317504.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  33. Ronceret A, Doutriaux M-P, Golubovskaya IN, Pawlowski WP: PHS1 regulates meiotic recombination and homologous chromosome pairing by controlling the transport of RAD50 to the nucleus. Proc Natl Acad Sci U S A. 2009, 106: 20121-20126. 10.1073/pnas.0906273106.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  34. Doutriaux MP, Couteau F, Bergounioux C, White C: Isolation and characterisation of the RAD51 and DMC1 homologs from Arabidopsis thaliana. Mol Gen Genet. 1998, 257: 283-291. 10.1007/s004380050649.

    Article  CAS  PubMed  Google Scholar 

  35. Abe K: Arabidopsis RAD51C gene is important for homologous recombination in meiosis and mitosis. Plant Physiol. 2005, 139: 896-908. 10.1104/pp.105.065243.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  36. Chen Z, Higgins JD, Hui JTL, Li J, Franklin FCH, Berger F: Retinoblastoma protein is essential for early meiotic events in Arabidopsis. EMBO J. 2011, 30: 744-755. 10.1038/emboj.2010.344.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  37. Grelon M, Vezon D, Gendrot G, Pelletier G: AtSPO11-1 is necessary for efficient meiotic recombination in plants. EMBO J. 2001, 20: 589-600. 10.1093/emboj/20.3.589.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  38. Stacey NJ, Kuromori T, Azumi Y, Roberts G, Breuer C, Wada T, Maxwell A, Roberts K, Sugimoto-Shirasu K: Arabidopsis SPO11-2 functions with SPO11-1 in meiotic recombination. Plant J. 2006, 48: 206-216. 10.1111/j.1365-313X.2006.02867.x.

    Article  CAS  PubMed  Google Scholar 

  39. Saracco SA, Miller MJ, Kurepa J, Vierstra RD: Genetic analysis of SUMOylation in Arabidopsis: conjugation of SUMO1 and SUMO2 to nuclear proteins is essential. Plant Physiol. 2007, 145: 119-134. 10.1104/pp.107.102285.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  40. Park HJ, Kim WY, Park HC, Lee SY, Bohnert HJ, Yun DJ: SUMO and SUMOylation in plants. Mol Cells. 2011, 32: 305-316. 10.1007/s10059-011-0122-7.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  41. Ishida T, Yoshimura M, Miura K, Sugimoto K: MMS21/HPY2 and SIZ1, two Arabidopsis SUMO E3 ligases, have distinct functions in development. PLoS ONE. 2012, 7: e46897-10.1371/journal.pone.0046897.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  42. Ling Y, Zhang C, Chen T, Hao H, Liu P, Bressan RA, Hasegawa PM, Jin JB, Lin J: Mutation in SUMO E3 ligase, SIZ1, disrupts the mature female gametophyte in Arabidopsis. PLoS ONE. 2012, 7: e29470-10.1371/journal.pone.0029470.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  43. Bergerat A, de Massy B, Gadelle D, Varoutas PC, Nicolas A, Forterre P: An atypical topoisomerase II from Archaea with implications for meiotic recombination. Nature. 1997, 386: 414-417. 10.1038/386414a0.

    Article  CAS  PubMed  Google Scholar 

  44. Bickel JS, Chen L, Hayward J, Yeap SL, Alkers AE, Chan RC: Structural maintenance of chromosomes (SMC) proteins promote homolog-independent recombination repair in meiosis crucial for germ cell genomic stability. PLoS Genet. 2010, 6: e1001028-10.1371/journal.pgen.1001028.

    Article  PubMed  PubMed Central  Google Scholar 

  45. Lichten M, Farmer S, San-Segundo PA, Aragón L: The Smc5–Smc6 complex is required to remove chromosome junctions in meiosis. PLoS ONE. 2011, 6: e20948-10.1371/journal.pone.0020948.

    Article  Google Scholar 

  46. Pebernard S: Nse1, Nse2, and a novel subunit of the Smc5-Smc6 complex, Nse3, play a crucial role in meiosis. Mol Biol Cell. 2004, 15: 4866-4876. 10.1091/mbc.E04-05-0436.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  47. McAleenan A, Cordon-Preciado V, Clemente-Blanco A, Liu IC, Sen N, Leonard J, Jarmuz A, Aragon L: SUMOylation of the alpha-kleisin subunit of cohesin is required for DNA damage-induced cohesion. Curr Biol. 2012, 22: 1564-1575. 10.1016/j.cub.2012.06.045.

    Article  CAS  PubMed  Google Scholar 

  48. Boavida LC, McCormick S: Temperature as a determinant factor for increased and reproducible in vitro pollen germination in Arabidopsis thaliana. Plant J. 2007, 52: 570-582. 10.1111/j.1365-313X.2007.03248.x.

    Article  CAS  PubMed  Google Scholar 

  49. Smyth DR, Bowman JL, Meyerowitz EM: Early flower development in Arabidopsis. Plant Cell. 1990, 2: 755-767. 10.1105/tpc.2.8.755.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  50. Mori T, Kuroiwa H, Higashiyama T, Kuroiwa T: Generative cell specific 1 is essential for angiosperm fertilization. Nat Cell Biol. 2006, 8: 64-71. 10.1038/ncb1345.

    Article  CAS  PubMed  Google Scholar 

  51. Ross KJ, Fransz P, Jones GH: A light microscopic atlas of meiosis in Arabidopsis thaliana. Chromosome Res. 1996, 4: 507-516. 10.1007/BF02261778.

    Article  CAS  PubMed  Google Scholar 

  52. Zhu J, Chen H, Li H, Gao JF, Jiang H, Wang C, Guan YF, Yang ZN: Defective in Tapetal development and function 1 is essential for anther development and tapetal function for microspore maturation in Arabidopsis. Plant J. 2008, 55: 266-277. 10.1111/j.1365-313X.2008.03500.x.

    Article  CAS  PubMed  Google Scholar 

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Acknowledgements

This work was supported by the National Science Foundation of China (31170269, U1201212,31370350), Education Department of Guangdong Province (2012CXZD0019), Guangdong Provincial Department of Science and Technology(S2012020011032, 2011A020201005)and Guangdong Province Universities and Colleges Pearl River Scholar Funded Scheme (2010).

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    1. Guangdong Key Lab of Biotechnology for Plant Development, College of Life Science, South China Normal University, Guangzhou, 510631, China

      Ming Liu, Songfeng Shi, Shengchun Zhang, Panglian Xu, Jianbin Lai, Yiyang Liu, Dongke Yuan, Yaqin Wang, Jinju Du & Chengwei Yang

    2. Vegetable Research Institute Guangdong Academy of Agriculture Sciences, Guangzhou, Guangdong, 510640, China

      Ming Liu

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    Authors’ contributions

    ML, SFS, SCZ, PLX, CWY analyzed and interpreted the data, and participated in manuscript preparation. YYL, DKY did the light and fluorescence microscopy. JL, YQW and JD helped with data analysis. CWY participated in the design and interpretation of experiments. All authors read and approved the final version of the manuscript.

    Ming Liu, Songfeng Shi, Shengchun Zhang, Panglian Xu contributed equally to this work.

    Electronic supplementary material

    12870_2013_1555_MOESM1_ESM.jpeg

    Additional file 1: Figure S1: Anther development at stages 3 and 5 in the wild-type and mms21-1. Early stages of pollen development in mms21-1 were comparable to wild-type. Anther stage 3 (A, C): cell division events occurred within the developing anther primordial. Anther stage 5 (B, D): the typical four-lobed anther morphology is established, and PMCs have formed in the center of each lobe. E, epidermis; MMC, microspore mother cell; Sp, sporogenou; T, tapetum. Bars = 20 μm. (JPEG 1 MB)

    12870_2013_1555_MOESM2_ESM.jpeg

    Additional file 2: Figure S2: Quantitative analysis of the number of irregular meiotic products in mms21-1 mutants. (A) Wild-type tetrads. (B-E) Irregular meiotic products in mms21-1 mutants. B,Monad. C, Dyad. D,Triad. E,Irregular tetrads. (F) Quantitative analysis of the number of irregular meiotic products in mms21-1 mutants. 354 mms21-1 meiotic products observed, 120 were normal tetrads(33.9%). 103 were irregular tetrads (29.1%), 36 triad (10.2%), 78 dyad(22.0%), 17 monad(4.8%). (JPEG 1 MB)

    12870_2013_1555_MOESM3_ESM.jpeg

    Additional file 3: Figure S3: Female gametophyte development is disrupted in mms21-1 mutants. (A) Mature embryo sac from wild-type plants. The positions of antipodal cells (white star), egg cell (black arrowheads), central cell nuclei (white arrowheads), and synergids (white star) are indicated. (B-D) Abnormal mature embryo sac from mms21-1 mutant plants. Embryo sacs containing one (B), two (C), or five (D) nuclei were observed in ovules in the mms21-1 mutants. Bars = 10 μm. (JPEG 449 KB)

    Additional file 4: Table S1: Primers used in this study. (DOC 90 KB)

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    Liu, M., Shi, S., Zhang, S. et al. SUMO E3 ligase AtMMS21 is required for normal meiosis and gametophyte development in Arabidopsis. BMC Plant Biol 14, 153 (2014). https://doi.org/10.1186/1471-2229-14-153

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