Open Access Research article

Genetic architecture of limit dextrinase inhibitor (LDI) activity in Tibetan wild barley

Yuqing Huang1, Shengguan Cai1, Lingzhen Ye1, Yong Han1, Dezhi Wu1, Fei Dai1, Chengdao Li2 and Guoping Zhang1*

Author Affiliations

1 Agronomy Department, Key Laboratory of Crop Germplasm Resource of Zhejiang Province, Zhejiang University, Hangzhou 310058, China

2 Department of Agriculture and Food, Western Australia, WA 6983, Australia

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BMC Plant Biology 2014, 14:117  doi:10.1186/1471-2229-14-117

Published: 1 May 2014



Limit dextrinase inhibitor (LDI) inhibits starch degradation in barley grains during malting because it binds with limit dextrinase (LD). There is a wide genetic variation in LDI synthesis and inactivation during barley grain development and germination. However, the genetic control of LDI activity remains little understood.


In this study, association analysis was performed on 162 Tibetan wild accessions by using LDI activity, 835 Diversity Arrays Technology (DArT) markers and single nucleotide polymorphisms (SNPs) of the gene HvLDI encoding LDI. Two DArT markers, bpb-8347, bpb-0068, and 31 SNPs of HvLDI were significantly associated with LDI activity, explaining 10.0%, 6.6% and 13.4% of phenotypic variation, respectively. Bpb-8347 is located on chromosome 6H, near the locus of HvLDI, and bpb-0068 is located on 3H.


The current results confirmed the locus of the gene controlling LDI activity and identified a new DArT markers associated with LDI activity. The SNPs associated with LDI activity may provide a new insight into the genetic variation of LDI activity in barley grains.

Limit dextrinase inhibitor (LDI); Genome-wide association study (GWAS); Single nucleotide polymorphism (SNP); Tibetan wild barley