|Read and assembly level statistics for T. versicolor interface transcriptomes|
|Sequencing||Z. mays||M. truncatula|
|Total raw sequence||2.73 Gbp||2.91 Gbp|
|Assembly length||12.77 Mbp||12.25 Mbp|
|Unigenes >500 bp||9,369||9,718|
|Min/Max length (bp)||197/3,115||197/3,265|
|N50||525 bp||536 bp|
|N50 >500 bp||731 bp||695 bp|
|Other Plant hits||2,975||2,086|
Low quality reads were filtered before assembly, and host sequences were filtered both before and after assembly. Unigenes remaining after removal of host plant and non-plant sequences were aligned with BLASTx to sequences detected in any other PPGP transcriptome library of Triphysaria versicolor (http://ppgp.huck.psu.edu/ webcite). Unigenes with less than 95% pairwise identity to either host or to other Triphysaria libraries were sorted further if a BLASTx search of the NR (http://www.ncbi.nlm.nih.gov webcite) database yielded alignments of 1e-10 or stronger. The remaining unclassified unigenes were submitted to OrthoMCL DB and InterProScan. Unigenes that remained unclassified after the final screen are called “no hit” unigenes.
Honaas et al.
Honaas et al. BMC Plant Biology 2013 13:9 doi:10.1186/1471-2229-13-9