Table 1

Read and assembly level statistics for T. versicolor interface transcriptomes
Host plant
Sequencing Z. mays M. truncatula
Total raw sequence 2.73 Gbp 2.91 Gbp
Reads Total 35,894,662 38,228,134
Host reads (401,352) (1,588,592)
Host filtered 35,493,310 36,639,542
Quality trimmed 26,947,737 27,325,845
Assembly
Assembly length 12.77 Mbp 12.25 Mbp
Unigenes Total 28,126 26,709
Unigenes >500 bp 9,369 9,718
Min/Max length (bp) 197/3,115 197/3,265
N50 525 bp 536 bp
N50 >500 bp 731 bp 695 bp
Annotation
Host unigenes (4,967) (7,785)
Non-plant unigenes (127) (329)
Triphysaria unigenes 23,032 18,595
Triphysaria hits 17,887 14,352
Other Plant hits 2,975 2,086
No hits 2,170 2,157

Low quality reads were filtered before assembly, and host sequences were filtered both before and after assembly. Unigenes remaining after removal of host plant and non-plant sequences were aligned with BLASTx to sequences detected in any other PPGP transcriptome library of Triphysaria versicolor (http://ppgp.huck.psu.edu/ webcite). Unigenes with less than 95% pairwise identity to either host or to other Triphysaria libraries were sorted further if a BLASTx search of the NR (http://www.ncbi.nlm.nih.gov webcite) database yielded alignments of 1e-10 or stronger. The remaining unclassified unigenes were submitted to OrthoMCL DB and InterProScan. Unigenes that remained unclassified after the final screen are called “no hit” unigenes.

Honaas et al.

Honaas et al. BMC Plant Biology 2013 13:9   doi:10.1186/1471-2229-13-9

Open Data