Additional file 1: Figure S1.

Unigene Pairwise Nucleotide Identity Plot. Sequence identity between unigenes considered in this study and reference EST sets (PlantGDB public ESTs, webcite) for the hosts Z. mays and M. truncatula. Triphysaria unigenes were aligned to the host reference to identify host contaminants and aligned to the reciprocal non-host reference sets to identify the incidental nucleotide pairwise identity. A whole plant normalized transcriptome assembly of Lindenbergia philippensis (a non-parasitic member of the Orobanchaceae) was used to determine the distribution of pairwise identity for a non-parasite to each host and to control for high unigene identity to host ESTs from potential cross contamination. A threshold of 95% was chosen to balance exclusion of host transcripts with retention of Triphysaria unigenes that had incident high identity to host ESTs.

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Honaas et al. BMC Plant Biology 2013 13:9   doi:10.1186/1471-2229-13-9