Table 4

Interactors of AGP6 and AGP11 identified by Yeast two-Hybrid library screening assays
AGI locus LBFCa Gene descriptionb Expression in mature pollenc Expression in pollen tubec Subcellular localizationd Other
AGP6 At1g62750 −1.66 Elongation factor G (SCO1) Apoplast [16]
At1g70810 −1.02 Calcium-dependent lipid-binding (CaLB domain) family protein [5-7] [5,6]
At5g05010 −1.00 Clathrin adaptor complexes medium subunit family protein (MUG13.13) [5-7] [5,6] Plasmodesmata [17], cytosol [18], plasma membrane [19] Predicted GPI-attachment [20], predicted secretory pathway [21], predicted beta-barrel TM domain [22]
At3g06650 −0.96 ATP-citrate lyase B-1 [7] [6]
At1g32640 −0.95 MYC-related transcriptional activator (MYC2) [5,6] No predicted binding site (cacatg), but two similar sites (acacaag) in AGP6 promoter region [23]
At1g67090 −0.93 Ribulose bisphosphate carboxylase small chain 1A (RBCS1A) [5-7] [5,6] Apoplast [16] Predicted secretory pathway [21]
At5g55070 −0.91 Dihydrolipoamide succinyltransferase [5], [6] [5,6] Mitochondrion [24]
At2g20580 −0.86 26S proteasome regulatory subunit N1 (RPN1A) [6] [6] Cytosol [18], plasmodesmata [17] One to five predicted alpha-helix TM domains [22], ubiquitin-binding subunit of proteasome complex [25]
At3g61190 −0.85 BON1-associated protein 1 (BAP1) [5], [7] [5,6] Plasma membrane [26]
At3g09440 −0.83 Heat shock protein 70-3 [5-7] [5,6] Cytosol [18], plasma membrane [27,28], cell wall [29], tonoplast [30], Golgi [31], apoplast [16] Predicted secretory pathway [21]
At5g21105 −0.77 L-ascorbate oxidase [6] [6] Plasmodesmata [17], cell wall [32] Predicted secretory pathway [21], predicted alpha-helix TM domains [22]
At5g28540 −0.52 Luminal-binding protein 1 (BIP1) [5-7] [5,6] Cell wall [29], cytosol [18], plasmodesmata [17], plasma membrane [27], ER [33], tonoplast [30], chloroplast [32] Predicted secretory pathway [21], one to two predicted alpha-helix TM domains [22]
At5g57560 −0.43 Xyloglucan endotransglucosylase/hydrolase 22 (TCH4, XTH22) [6] Cell wall [24] Predicted secretory pathway [21], predicted alpha-helix TM domain and predicted beta-barrel TM domain [22]
At5g15090 0.55 Mitochondrial outer membrane protein porin 2 (VDAC3) [6] [6] Plasma membrane [19,28], cell wall [29] Predicted beta-barrel TM domain [22]
At1g16920 0.71 Ras-related small GTP-binding protein (RABA1b) [7]
At3g18040 0.75 Mitogen-activated protein kinase 9 (MAPK9) [5-7] [5,6] Predicted GPI-attachment [20], predicted alpha-helix TM domain [22]
At4g05320 0.84 Polyubiquitin 10 (UBQ10) [5-7] [5,6]
At2g20990 0.87 Synaptotagmin A (SYTA) [7] [6] Endosome [34], plasma membrane [19,28,35], ER [32] Predicted secretory pathway [21], predicted alpha-helix TM domain [22]
At1g26630 0.89 Eukaryotic translation initiation factor 5A-2 (FBR12) [5-7] [5,6] Predicted GPI-attachment [20]
At3g10740 0.92 Alpha-L-arabinofuranosidase 1 (ASD1) [6], [7] [6] Apoplast [16], cell wall [36] Predicted secretory pathway [21], one to two predicted alpha-helix TM domains [22]
At5g11200 1.22 DEAD-box ATP-dependent RNA helicase 56 [6] [5,6] Cell wall [29], plasmodesmata [17]
At1g31150 2.50 Uncharacterized protein [7] Predicted secretory pathway [21], predicted alpha-helix TM domain [22]
AGP11 At3g47810 −1.43 MAG1 Homolog of yeast retromer subunit VPS29 [5-7] [5,6] Multivesicular body [37], endosome [38] Mediator of protein targeting to vacuole [39]
At1g42990 −0.97 bZIP transcription factor 60 (BZIP60) [5-7] [5,6] Predicted GPI-attachment [20], predicted alpha-helix TM domain [22]
At1g32640 −0.95 MYC-related transcriptional activator (MYC2) [5,6] Two predicted binding sites in AGP11 promotor region [23]
At2g19070 −0.63 Spermidine hydroxycinnamoyl transferase (SHT) Tapetum-specific [40], involved in exine formation [40,41]
At2g30020 0.74 Putative protein phosphatase 2C 25 [5-7] [5,6] Nucleus [42,43], cytoplasm [43]

aagp6 agp11 to wild type lower bound of fold change.

b TAIR gene annotations [14].

c in published microarray experiments.

d empirically determined in published references.

Costa et al.

Costa et al. BMC Plant Biology 2013 13:7   doi:10.1186/1471-2229-13-7

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