Yeast two-hybrid experiments with candidate genes selected from the library screening. YSD media -tryptophane, -leucine, -histidine 1 mM 3-Amino-1,2,4-triazole. Empty AD and BD vectors were used as negative controls. (A) Assays with AGP6 protein core as bait and CaLB domain family protein (At1g70810), MAPK9 (At3g18040), UBQ10 (At4g05320) and FBR12 (At1g26630) as preys. (B) Assays with AGP11 protein core as bait and AP2C1 (At2G30020) as prey.
Costa et al. BMC Plant Biology 2013 13:7 doi:10.1186/1471-2229-13-7