Comparative expression profiling of miRNA during anther development in genetic male sterile and wild type cotton
1 State Key Laboratory of Cotton Biology, Institute of Cotton Research of CAAS, Anyang, 455000 Henan, P. R. China
2 College of Agronomy, Northwest A&F University, Yangling, 712100 Shaanxi, P. R. China
3 Department of biology, New Mexico State University, Montreal 880033, USA
BMC Plant Biology 2013, 13:66 doi:10.1186/1471-2229-13-66Published: 19 April 2013
Additional file 1:
The number of total miRNA and siRNA in six small RNA libraries.
Format: XLSX Size: 35KB Download file
Additional file 2:
Cotton conserved miRNA families in six small RNA libraries.
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Additional file 3:
The number of significant differentially expressed cotton conserved miRNA in the six small RNA.
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Additional file 4:
Length distribution of small RNAs in degradome library.
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Additional file 5:
The number of distinct transcripts targeted by unique miRNAs detected in degradome library.
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Additional file 6:
GO analysis of miRNA target genes identified in the WT and GMS mutant anthers representing three stages of development.
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Additional file 7:
The significant sites on the t-plots.
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Additional file 8:
Comparison of the qRT-PCR results of the identified cotton miRNAs with the Solexa sequencing results of the corresponding miRNAs. (a), (c), (e) Solexa sequencing results of miRNAs; (b), (d), (f) qRT-PCR results of miRNAs. F-1 and S-1: meiosis stage of the wild and mutant anthers; F-2 and S-2: tetrad stage of the wild and mutant anthers; F-3 and S-3: uninucleate microspore stage of the wild and mutant anthers. Relative expression levels (R.E.Ls) were calculated using 18S as a control.
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Additional file 9:
Novel miRNAs identified from six small RNA libraries.
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Additional file 10:
The number of significant differentially expressed novel cotton miRNA in the six libraries.
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Additional file 11:
Target plots (t-plots) of identified novel miRNA (Mar-F-1-m0031) targets using degradome sequencing. The abundance of each signature is plotted as a function of its position in the transcript. The red colored italicized nucleotide on the target transcript from the 3′ end indicates the cleavage site detected in the degradome library.
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Additional file 12:
Measurment of IAA contents in the uninucleate microspore stage of WT and GMS mutant anthers using high performance liquid chromatography. F-3 and S-3: uninucleate microspore stage wild type and mutant anthers.
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Additional file 13:
The qRT-PCR results of cytochrome c in anthers of the WT and GMS mutant.
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