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Open Access Research article

Suppression of plant defense responses by extracellular metabolites from Pseudomonas syringae pv. tabaci in Nicotiana benthamiana

Seonghee Lee, Dong Sik Yang, Srinivasa Rao Uppalapati, Lloyd W Sumner and Kirankumar S Mysore*

Author Affiliations

The Samuel Roberts Noble Foundation, Plant Biology Division, Ardmore, OK, 73401, USA

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BMC Plant Biology 2013, 13:65  doi:10.1186/1471-2229-13-65

Published: 18 April 2013

Additional files

Additional file 1: Table S1:

List of extracellular metabolites secreted from P. syringae pv. tabaci using UHPLC-qTOF-MS.

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Additional file 2: Figure S1:

Expression patterns of PR5, PDF1.2, ABA1, HSR203J and COI1 genes after treating with MG or Pstab extracts in N. benthamiana. The expression level of PR5, PDF1.2, ABA1, HSR203J and COI1 as determined by qRT-PCR after treatment of Pstab extracts. Gene expression analyses were performed using three biological replications. Bars represent the means ± standard deviation (SD). Same letters above bars indicate no statistically significant difference (P<0.05) among plant genotypes for a given time point using one-way ANOVA followed by LSD test analysis. The qRT-PCR data were normalized to NbActin transcript and shown as relative to that of target gene expressions in 0 hr N. benthamiana leaves without any treatment.

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