Table 1

Summary of the positive identifications of the pea leaf proteins eluted from the GSNO vinyl sulfone resin
Protein score C.I.% Peptide count Protein species MW (kDa) pI ID UniProtKB Comments
Chloroplastidic enzymes
Fructose-1,6-bisphosphatase P46275 Benson-Calvin cycle enzyme.
100 9 with transit peptide 42.3 5.00 Regulated by thioredoxin.
100 9 mature protein 35.3 Reported as nitrated [88].
100 8 nicked protein 34.0 5.02 Reported as S-nitrosylated [43].
Pisum sativum
100 11 Phosphoribulokinase 39.2 5.40 P93681 Benson-Calvin cycle enzyme.
Pisum sativum
Regulated by thioredoxin.
Reported as nitrated [88].
Ferredoxin:NADP reductase P10933 It catalyzes the reduction of
100 9 with transit peptide 40.2 8.60 NADP+ to NADPH using the electrons provided by reduced ferredoxin.
100 8 mature protein 33.0 6.06
Pisum sativum
Reported as nitrated [89].
100 7 Hypothetical protein 42.8 8.19 Q9SA52 According to the UniprotKB:
(predicted as chloroplastidic) sugar epimerase family.
Arabidopsis thaliana Subcellular location: chloroplast.
Proteasome Reported as S-nitrosylated [65].
100 6 26S proteasome alpha-3 chain 27.5 6.11 O24362 Involved in the structure of the proteasome particle.
Spinacia oleracea
100 8 Proteasome subunit alpha type-7 27.2 6.86 Q9SXU1 Involved in the structure of the proteasome particle.
Cicer arietinum
100 10 Proteasome subunit alpha type-6 27.5 5.83 O48551 Involved in the structure of the proteasome particle.
Glycine max
100 7 Proteasome subunit alpha type-2-A 25.7 5.53 O23708 Involved in the structure of the proteasome particle.
Arabidopsis thaliana
100 6 Putative beta4 proteasome subunit 14.6 6.40 Q93X32 Protease subunit.
Nicotiana tabacum
Regulatory proteins
97.1 9 Putative F-box protein 38.1 9.80 Q9FVT4 F-box proteins have been associated to ubiquitination, signal transduction and regulation of the cell-cycle.
Arabidopsis thaliana
Reported as nitrated [88].
98.8 8 GTP-binding protein 25.0 5.70 Q08147 Small GTP-binding proteins belong to a superfamily involved in the regulation as biotimers of a wide variety of cell functions.
Pisums sativum,
Reported as S-nitrosylated in mammals [62,63].
99.7 12 CRK1 protein. 66.7 9.26 Q9LDC1 Cytokinine Regulated Kinase 1.
Beta vulgaris
98.8 11 Inducer of CBF expression 1 protein 60.8 6.51 Q1HIU3 C-repeat Binding Factor binds to CRT/DRE which actives many downstream genes that confer chilling and freezing tolerance to plants.
Populus suaveolens.
100 15 Heat shock protein Hsp70 75.8 5.19 Q1SKX2 Hsp70s are part of the machinery for protein folding and protection from stress.
Medicago truncatula
Reported as S-nitrosylated [37].
99.7 10 S-adenosyl-L-methionine: 41.6 6.21 Q9LS20 It catalyzes the formation of the methyl ester of the salicylic acid, involved in the localized and systemic defense responses of plants.
salicylic acid carboxyl methyltransferase
Arabidopsis thaliana

Begara-Morales et al.

Begara-Morales et al. BMC Plant Biology 2013 13:61   doi:10.1186/1471-2229-13-61

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