Chromatographic fractionation. Profile at three wavelengths (280 nm for protein and both 340 nm and 550 nm for nitrosothiol) of the pea leaf fractions eluted from the GSNO vinyl sulfone resin by 200 mM NaCl (gray) and 1500 mM NaCl (green) in 50 mM HEPES pH 7.4. Insert, SDS-PAGE of the fractions labeled as a, b, c, d and e, the crude extract (X) and the eluted fraction from the column during injection of mililiter 50 of the sample (S). The numbers on the left side of the SDS-PAGE indicate the relative molecular masses in kDa. Proteomic studies were carried out on fraction c.
Begara-Morales et al. BMC Plant Biology 2013 13:61 doi:10.1186/1471-2229-13-61