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Open Access Research article

Identification and characterization of lysine-rich proteins and starch biosynthesis genes in the opaque2 mutant by transcriptional and proteomic analysis

Mo Jia1, Hao Wu1, Kasi L Clay1, Rudolf Jung2, Brian A Larkins3 and Bryan C Gibbon1*

Author Affiliations

1 Department of Biology, Baylor University, One Bear place #97388, Waco, TX 76798, USA

2 Pioneer Hi-Bred International, Inc., Johnston, IA 50131, USA

3 Department of Plant Sciences, University of Arizona, Tucson, AZ 85721, USA

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BMC Plant Biology 2013, 13:60  doi:10.1186/1471-2229-13-60

Published: 12 April 2013

Abstract

Background

The opaque2 mutant is valuable for producing maize varieties with enhanced nutritional value. However, the exact mechanisms by which it improves protein quality and creates a soft endosperm texture are unclear. Given the importance of improving nutritional quality in grain crops, a better understanding of the physiological basis for these traits is necessary.

Results

In this study, we combined transcript profiling and proteomic analysis to better understand which genes and proteins are altered by opaque2 in the W64A inbred line. These analyses showed that the accumulation of some lysine-rich proteins, such as sorbitol dehydrogenase and glyceraldehyde3-phosphate dehydrogenase, was increased in mature kernels and may contribute substantially to the lysine content of opaque2 endosperm. Some defense proteins such as beta-glucosidase aggregating factor were strongly down regulated and may be regulated directly by opaque2. The mutant also had altered expression of a number of starch biosynthesis genes and this was associated with a more highly crystalline starch.

Conclusions

The results of these studies revealed specific target genes that can be investigated to further improve nutritional quality and agronomic performance of high lysine maize lines, particularly those based on the presence of the opaque2 mutation. Alteration of amylopectin branching patterns in opaque2 starch could contribute to generation of the soft, starchy endosperm.

Keywords:
Opaque endosperm; Opaque2; Quality protein maize; Starch biosynthesis; Protein quality