Phylogenetic tree of BRAMI-1 elements from Brassica species and ATTIRTA-1 fromA. thaliana. Relatively intact MITE members showing 80% similarity to the characteristic MITE structure were used for the analysis. A total of 528 BRAMI-1 members including 401, 123, and 4 from B. rapa (red), B. oleracea (blue), and B. napus (black), respectively, and 34 ATTIRTA-1 members (green) were compared. Sequence alignment was conducted using ClustalW and then the phylogenetic tree was generated using the neighbor joining method with 500 bootstrap replicates.
Sampath et al. BMC Plant Biology 2013 13:56 doi:10.1186/1471-2229-13-56