Table 2

Degenerate primers for cloning of energy-related genes in litchi pericarp
Gene Forward primer (5’ to 3’) Reverse primer (5’ to 3’) Product (bp)
AtpB GGGCCGTGGCCatgwsngcnac TCGGGCAGGCCGtcnarytcncc 1188
AOX1 GGAGGACGGCACCGAntggmmntg GCGTCCTTGGGCAGCCkccartartcda 676
UCP1 ACCATCGCCCGGGARGARGGNNT AGGTGCCCAGCCGGSYRAARTTNGG 652
AAC1 GCCGCCGCCccnathgarmg CGCCGCCGGAGCcrtayttyttnc 850
SnRK2 GGCGCCGGCAACttyggngtngc AGCTCCCTGGGCAGGttyttnarraac 773

AtpB, ATP synthase β subunit; AOX1, alternative oxidase 1; UCP1, uncoupling mitochondrial protein 1; AAC1, ADP/ATP carrier 1; SnRK2, sucrose non-fermenting-1-related kinase 2.

Wang et al.

Wang et al. BMC Plant Biology 2013 13:55   doi:10.1186/1471-2229-13-55

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