Melting curve analyses of two different qRT-PCR reactions. The negative first derivative of the change in fluorescence is plotted as a function of temperature. The single peak for each primer pair indicates the presence of only one PCR product. A) Melting curves for Mal d 1.01 (red), Mal d 1.02 (blue) and Mal d 1.03B (green) amplicons. B) Melting curves for Mal d 1.11A (red), Mal d 1.07 (blue) and Mal d 1.06A (green) amplicons.
Pagliarani et al. BMC Plant Biology 2013 13:51 doi:10.1186/1471-2229-13-51