Additional file 1.

Figure S1. Sequence alignment of the putative polypeptides derived from the 11 safflower CtFAD2 and orthologous plant FAD2s. atDES, AAM61113.1; lcDES, ACR15954.1; pfOH:DES, AAC32755.1; plOH, ABQ01458.1; coCONJ, AAK26632.1; haACET, ABC59684.1; rhACET, AAO38035.1; dsACET, AAO38036.1; caACET, ABC00769.1; cpEPOX, CAA76156.1; slEPOX, AAR23815.1; dcACET, AAO38033.1; dcDES:OH, AAK30206.1; fvACET,AAO38034.1; hhACET, AAO38031.1; boDES, AAC31698.1; haDES-2, AAL68982.1; haDES-3, AAL68983.1; haDES-1, AAL68981.1; ntDES,AAT72296.2; oeDES, AAW63040; siDES, AAF80560.1; ghDES-1, CAA65744.1; ptDES, XP_002297660.1; rcOH, AAC49010.1; cpDES, AAS19533.1; ghDES-4, AAQ16653.1; ghDES-2, CAA71199.1; jcDES, ADB93805.1; luDES,ACF49507.1. Figure S2. Mass spectral identification of DMOX derivatives of C18:2Δ9(Z),12(E) and C18:2Δ9(Z),12(Z) from S. cerevisae expressing safflower CtFAD2-11. Figure S3. Mass spectral identification of DMOX derivatives of crepenynic acid (9-octadecen-12-ynoic acid) from N. benthamiana leaves transiently expressing safflower CtFAD2-11 (A). methyl crepenynate standard. Table S1. Oligonucleotide primers used in the 3′RACE of multiple CtFAD2 genes in safflower Table S2. Oligonucleotide primers used for amplification of the entire coding region of CtFAD2 genes in safflower. Table S3. Oligonucleotide primers used for the amplification of 5′UTR intron of CtFAD2 genes in safflower. Table S4. Oligonucleotide primers used for RT-qPCR in the expression profile study of safflower CtFAD2 genes.

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Cao et al. BMC Plant Biology 2013 13:5   doi:10.1186/1471-2229-13-5