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Open Access Research article

Expression profiling of tomato pre-abscission pedicels provides insights into abscission zone properties including competence to respond to abscission signals

Toshitsugu Nakano, Masaki Fujisawa, Yoko Shima and Yasuhiro Ito*

Author Affiliations

Food Biotechnology Division, National Food Research Institute, NARO, 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan

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BMC Plant Biology 2013, 13:40  doi:10.1186/1471-2229-13-40

Published: 9 March 2013

Additional files

Additional file 1:

Genes up-regulated in AZ compared with Prox.

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Additional file 2:

Genes up-regulated in AZ compared with Dis.

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Additional file 3:

Validation of the microarray data by RT-PCR assays on genes that exhibited AZ preferential expression by the microarray assays. Transcript levels of 10 genes in the pedicel regions, AZ, Prox and Dis, were compared for the microarray and RT-PCR assays. The results obtained by the two methods showed good consistency for all the examined genes. As an internal control (Ctrl) for the RT-PCR assays, SAND (SGN-U316474) was used [96].

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Additional file 4:

Genes down-regulated in AZ compared with non-AZ (Prox and Dis).

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Additional file 5:

Genes up-regulated in Prox compared with Dis.

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Additional file 6:

Genes up-regulated in Dis compared with Prox.

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Additional file 7:

Validation of the microarray data by RT-PCR on genes differentially expressed between Prox and Dis. Transcript levels of 11 genes in the pedicel regions, AZ, Prox and Dis, were compared with the microarray assays and RT-PCR assays. The results obtained by the two methods showed good consistency for all the examined genes. As an internal control (Ctrl) for the RT-PCR assays, SAND (SGN-U316474) was used [96].

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Additional file 8:

Frequency of flower pedicel abscission after removal of the flower. Anthesis flowers were removed from the pedicels to induce abscission and then the number of abscised pedicels was counted. In total, 206 flower pedicels were used for the analysis.

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Additional file 9:

Expression of Bl, GOB, Ls, LeWUS and TAPG4 in the AZ of a tomato flower pedicel at anthesis. When tissue sections of the AZ of a flower pedicel at anthesis were hybridized with the DIG-labeled antisense probes for Bl, GOB, Ls LeWUS and TAPG4, the hybridization signals were detected in tube-like vascular cells but no specific signals were detected when their sense probe were examined. The signals for each gene are indicated by arrowheads.

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Additional file 10:

The expression patterns of TA50096_4081 (an ARF9 homologue) and AK319758 (an IAA29 homologue) in tomato flower anthesis pedicels. The transcript level of AK319758 was the highest in Dis and decreased gradually toward Prox; TA50096_4081 was significantly higher in Prox compared to Dis. The expression signal intensity was detected by microarray assays and error bars indicate standard deviation of biological triplicates for the assay.

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Additional file 11:

Sequences of primers used in this study.

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