Table 3

Types of induced mutation inFatAandSADand mutation frequency
Target genes Function Fragment Size (bp) Identified mutations Mutation frequency (1/kb)
Missense Stop codon Silent Intronic Promoter region Total
FatA Fatty acid biosynthesis 1,881 3 1 6 1 2 12 1/783
SAD Fatty acid biosynthesis 619 6 0 8 0 0 14 1/221
Total 2,500 9 1 13 1 2 26 1/480

The mutation frequency for each amplicon is calculated as follows:

[(size of the amplicon) × (total number of samples screened)] / (total number of identified mutants). The average mutation frequency was estimated to be one mutation per 480 kb.

Kumar et al.

Kumar et al. BMC Plant Biology 2013 13:38   doi:10.1186/1471-2229-13-38

Open Data