Open Access Research article

Selective defoliation affects plant growth, fruit transcriptional ripening program and flavonoid metabolism in grapevine

Chiara Pastore1, Sara Zenoni2, Marianna Fasoli2, Mario Pezzotti2, Giovanni Battista Tornielli2* and Ilaria Filippetti1

Author Affiliations

1 Department of Fruit Tree and Woody Plant Science, University of Bologna, Viale Fanin, 46, 40126, Bologna, Italy

2 Department of Biotechnology, University of Verona, Strada Le Grazie 15, 37134, Verona, Italy

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BMC Plant Biology 2013, 13:30  doi:10.1186/1471-2229-13-30

Published: 22 February 2013

Additional files

Additional file 1:

Effects of pre-bloom (PB) and veraison (V) defoliation compared to an untreated control (C) on Sangiovese berry weight (A), soluble solids (B) and titratable acidity (C). Vertical bars indicate the standard deviation of the mean (n = 3 biological replicates).

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Additional file 2:

Differentially-expressed genes displaying a two-fold or greater difference in transcript abundance between C and PB berries at EV and H, and between C and V berries at EV. For each gene, we show the PB/C and V/C fold-change (FC), the description, the GO functional category and the increase (↑) or decrease (↓) in expression between BV and EV in C berries.

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Additional file 3:

Common differentially-expressed genes between C and PB and C and V berries at EV, displaying a two-fold or greater change in transcript abundance and belonging to selected functional categories. For each gene, we show the fold-change (FC) at EV, the description and the increase (↑) or decrease (↓) in expression between BV and EV in C berries.

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Additional file 4:

Specific differentially-expressed genes between C and PB and C and V berries at EV, displaying a two-fold or greater change in transcript abundance and belonging to selected functional categories. For each gene, we show the fold-change (FC) at EV, the description and the increase (↑) or decrease (↓) in expression between BV and EV in C berries.

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Additional file 5:

Differentially-expressed genes during berry development displaying a two-fold or greater change in transcript abundance between EV and BV or EV and H, in C, PB and V berries. For each gene, we show the description, the EV/BV and H/BV fold-change (FC) and the cluster number. Data obtained for C, PB and V berries are listed in three separate worksheets.

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Additional file 6:

Representative expression profiles of the eight clusters.

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Additional file 7:

Differentially-expressed genes during berry development displaying a three-fold or greater change in transcript abundance between EV and BV or EV and H, in C, PB and V berries. For each gene, we show the description, the EV/BV and H/BV fold-change (FC), the cluster number or the non-modulation (NM) in each treatment, and the effect on gene expression in comparison to C berries. Genes commonly affected in the PB and V berries, genes differentially affected in the PB and V berries, genes specifically affected in PB berries and genes specifically affected in V berries are listed in four separate worksheets.

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Additional file 8:

Genes belonging to selected functional categories, differentially-expressed during berry development, displaying a three-fold or greater change in transcript abundance between EV and BV or EV and H, in C, PB and V berries. For each gene, we show the description and the effect on gene expression in comparison to C berries. Downregulation, downregulation advanced, upregulation delayed and no upregulation effects compared to C berries are indicated in green; upregulation, upregulation advanced, downregulation delayed and no down regulation effects compared to C berries are indicated in red; gray color indicates no difference compared to C.

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Additional file 9:

Real time RT-PCR validation of MYBA1/A2 (VIT_02s0033g00410 and VIT_02s0033g00390), F3Hb (VIT_17s0000g07210), DFR (VIT_18s0001g12800), and MYBPA1 (VIT_15s0046g00170) expression profiles in pre-bloom defoliated (PB), veraison defoliated (V) and control (C) berries during ripening. The amplification of MYBA1 and MYBA2 transcripts was performed using a primer pair that recognizes both sequences [47]. Expression profiles measured by real time RT-PCR were determined by calculating the relative expression ratio value for each stage relative to the BV stage. Real time RT-PCR data are reported as means ± SE of three biological replicates, obtained using elongation factor 1 (VIT_06s0004g03220) for normalization.

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Additional file 10:

List of the primers used for real time RT-PCR.

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