Table 2

Phobius transmembrane and signal sequence prediction for members of the Arabidopsis, Populus and Physcomitrella IRX10 family of proteins
Protein Amino acid Prediction Protein Amino acid Prediction
AtIRX10 1-21 Signal AtIRX10-L 1-19 Signal
1-5 N-region 1-2 N-region
6-16 H-region 3-13 H-region
17-21 C-region 14-21 C-region
22-412 Non-cytoplasmic 22-415 Non-cytoplasmic
PtGT47A-1 1-19 Signal PtGT47A-2 1-19 Signal
1-2 N-region 1-2 N-region
3-14 H-region 3-14 H-region
15-19 C-region 15-19 C-region
20-412 Non-cytoplasmic 20-413 Non-cytoplasmic
PtGT47D-1 and PtGT47D-4 1-5 Cytoplasmic PpGT47A 1-31 Cytoplasmic
6-26 Transmembrane 32-51 Transmembrane
27-417 Cytoplasmic 52-449 Non-cytoplasmic

IRX10 related protein sequences from Arabidopsis, Populus and Physcomitrella were analysed using the Phobius programme (http://www.phobius.sbc.su.se webcite) for the presence of N-terminal signal sequences and the locations of the hydrophobic predicted transmembrane core (H-region) flanked by the N- and C- regions.

Hörnblad et al.

Hörnblad et al. BMC Plant Biology 2013 13:3   doi:10.1186/1471-2229-13-3

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