Knockout of the Physcomitrella PpGT47A gene does not cause a significant change in cell wall monosaccharide composition.A) Schematic drawing of the PpGT47A gene and the knock-out construct which was introduced via homologous recombination to obtain knockout mutants in Physcomitrella. ** corresponds to a truncated version of the E4 sequence and * corresponds to a truncated version of the 3′UTR sequence which are indicated in the upper genomic sequence. B) Monosaccharide analysis of gametophore cell walls from Physcomitrella wild-type and 4 independent gt47a knockout mutants. Data were obtained from 3 technical replicates of two pooled samples for the wild-type and knockout Ppgt47a:3, and three pooled samples for knockouts Ppgt47a:2; Ppgt47a:5 and Ppgt47a:11 .
Hörnblad et al. BMC Plant Biology 2013 13:3 doi:10.1186/1471-2229-13-3