Effect of WT, atskip-i, GmGBP1-ox, myb33 and GmGBP1-ox/myb33plants. (A) Phenotypes of plants in LDs and SDs. Photos at 25 days after germination in LDs; Photos at 42 days after germination in SDs. Bars = 1cm. (B) Expression level of flowering related gene in Arabidopsis. RT-PCR analysis for AtCO and AtFT in WT, atskip-i, GmGBP1-ox plants in LDs; RT-PCR analysis for AtFLC for plants in SDs. Samples were prepared from rosette leaves before bolting in LDs or SDs. (C) Expression level of AtLFY in plants. Samples were prepared from rosette leaves before bolting in LDs. (D) Flowering time of plants in LDs and SDs. Flowering time was measured as the total number of rosette leaves or days to bolting. Data were from more than 20 individuals for each genotype ± SD. WT plants compared with atskip-i, GmGBP1-ox, myb33 and GmGBP1-ox/myb33 plants, respectively. * showed significant to WT plant at 0.05 level (P < 0.05) by t-test; ** showed significant to WT plant at 0.01 level (P < 0.01) by t-test.
Zhang et al. BMC Plant Biology 2013 13:21 doi:10.1186/1471-2229-13-21