Calcium is accumulated in the cytosol after wounding. Leaves that constitutively express cytosolic aequorin are incubated over-night in 10 μM CTZ, placed into a luminometer and then wounded. Luminescence was measured and depended on the amount of calcium that binds to aequorin and thus reflects cytosolic calcium influx. In the control, leaves were infiltrated with water, otherwise with the mentioned concentrations of calcium inhibitors.
Beneloujaephajri et al. BMC Plant Biology 2013 13:160 doi:10.1186/1471-2229-13-160