Open Access Research article

Localization of sesquiterpene formation and emission in maize leaves after herbivore damage

Tobias G Köllner1*, Claudia Lenk2, Christiane Schnee13, Sabrina Köpke1, Peter Lindemann2, Jonathan Gershenzon1 and Jörg Degenhardt2

Author Affiliations

1 Max Planck Institute for Chemical Ecology, Hans-Knöll-Strasse 8, D-07745, Jena, Germany

2 Institute of Pharmacy, Martin-Luther-University Halle-Wittenberg, Hoher Weg 8, D-06120, Halle/Saale, Germany

3 Current address: Friedrich Löffler Institute, Naumburger Strasse 96a, Jena, 07743, Germany

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BMC Plant Biology 2013, 13:15  doi:10.1186/1471-2229-13-15

Published: 30 January 2013

Additional files

Additional file 1: Figure S1:

Comparison of sesquiterpene emission from intact plants as measured by standard headspace volatile collection vs. SPME collection from macerated tissue. The results of the two methods are directly correlated with each other in terms of the amount emitted and the relative proportion of individual sesquiterpenes. (A) Maize plants were treated with Spodoptera littoralis caterpillars (see Methods). Headspace collection was carried out using a standard system as described in the Methods. For maceration-SPME, the plants were frozen right after volatile collection and ground in liquid nitrogen. Sesquiterpenes released from plant powder were collected using SPME. Samples were analyzed using GC-MS and the peak areas for (E)-β-farnesene + (E)-α-bergamotene (TPS10 products) and (E)-β-caryophyllene (TPS23 product) were used for comparison. To cover a broad range of volatile emission, plants of the inbred line B73 (which are known to emit only low amounts of sesquiterpenes) as well as plants of the hybrid Delprim (which are described as strong emitters) were used in this experiment. (B) GC-MS traces of sesquiterpenes emitted from an intact herbivore-induced Delprim plant (left) and sesquiterpenes released from powder of the same plant (right). 1, (E)-β-caryophyllene ; 2, (E)-α-bergamotene ; 3, (E)-β-farnesene ; 4, α-humulene; 5, β-bisabolene ; 6, β-sesquiphellandrene.

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