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Open Access Highly Accessed Research article

Perception of soft mechanical stress in Arabidopsis leaves activates disease resistance

Lehcen Benikhlef1, Floriane L’Haridon1*, Eliane Abou-Mansour1, Mario Serrano1, Matteo Binda1, Alex Costa2, Silke Lehmann1 and Jean-Pierre Métraux1

Author Affiliations

1 Department of Biology, University of Fribourg, 10 chemin du Musée, CH-1700, Fribourg, Switzerland

2 Department of Biosciences, University of Milan, via G. Celoria 26, 20133, Milan, Italy

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BMC Plant Biology 2013, 13:133  doi:10.1186/1471-2229-13-133

Published: 13 September 2013

Abstract

Background

In a previous study we have shown that wounding of Arabidopsis thaliana leaves induces a strong and transient immunity to Botrytis cinerea, the causal agent of grey mould. Reactive oxygen species (ROS) are formed within minutes after wounding and are required for wound–induced resistance to B. cinerea.

Results

In this study, we have further explored ROS and resistance to B. cinerea in leaves of A. thaliana exposed to a soft form of mechanical stimulation without overt tissue damage. After gentle mechanical sweeping of leaf surfaces, a strong resistance to B. cinerea was observed. This was preceded by a rapid change in calcium concentration and a release of ROS, accompanied by changes in cuticle permeability, induction of the expression of genes typically associated with mechanical stress and release of biologically active diffusates from the surface. This reaction to soft mechanical stress (SMS) was fully independent of jasmonate (JA signaling). In addition, leaves exposed soft mechanical stress released a biologically active product capable of inducing resistance to B. cinerea in wild type control leaves.

Conclusion

Arabidopsis can detect and convert gentle forms of mechanical stimulation into a strong activation of defense against the virulent fungus B. cinerea.