Subcellular localizations of Physcomitrella NDA type proteins. GFP was fused to the C-terminus of NDA1 and NDA2 from Physcomitrella patens (Pp). Additionally, the last 10 amino acids were fused at the C-terminus of GFP. Subcellular targeting was analyzed in both Arabidopsis cell suspension, onion epidermal tissue and Physcomitrella protonemal tissue along with RFP tagged mitochondrial or peroxisomal controls. The protein accession number, the location of GFP and the number of amino acids used are shown for each construct. Mitochondria (M) and peroxisomes (Px) are indicated. M-RFP - mitochondrial RFP, Px-RFP - peroxisomal RFP. Scale bar indicates 10 μm.
Xu et al. BMC Plant Biology 2013 13:100 doi:10.1186/1471-2229-13-100