PCR analysis of 5 S organization. Schemes of separate (A) and linked (B) configuration of 5 S rDNA. Positions of primers are marked with arrowheads. The lines below indicate putative PCR products. (C) Analysis of separate organization using the 5SgF/5SgR primer pair. The arrow indicates position of the band extracted for subsequent cloning. Asterisk – position of a 5 S monomer (120 bp). (D) Analysis of linked organization using the 5SgF/26SPr1 primer pair. PCR products were separated in 1.2% agarose gels and detected by ethidium bromide fluorescence.
Garcia et al. BMC Plant Biology 2012 12:95 doi:10.1186/1471-2229-12-95