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Open Access Research article

Expression of 5 S rRNA genes linked to 35 S rDNA in plants, their epigenetic modification and regulatory element divergence

Sònia Garcia1, Lucie Crhák Khaitová2 and Aleš Kovařík2*

Author Affiliations

1 Laboratori de Botànica, Facultat de Farmàcia, Universitat de Barcelona, Av. Joan XXIII s. n., Barcelona, Catalonia, 08028, Spain

2 Institute of Biophysics, Academy of Sciences of the Czech Republic, Královopolská 135, Brno, CZ-6125, Czech Republic

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BMC Plant Biology 2012, 12:95  doi:10.1186/1471-2229-12-95

Published: 20 June 2012

Additional files

Additional file 1:

Sequencing of 5 S oligomers from A. tridentata. Alignment of sequenced clones. Coding regions are in bold letters. Boxes A and C are in yellow shading. The TATA box and termination signals are in red and blue, respectively. Asterisks indicate mutations.

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Open Data

Additional file 2:

Alignment of long 5 S-IGS1 clones from A. absinthium. Alignment of 3 cDNA and 2 genomic (gDNA) clones containing 5 S genic and intergenic sequences. Termination signals are underlined.

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Open Data

Additional file 3:

Structure of the 26 S-5 S intergenic spacer. Alignment of genomic clones. The first ~30 nucleotides represent the 3’end of the 26 S gene. The last nucleotide belongs to the 5 S coding region. Strand reading the 35 S gene is shown; 5 S is encoded by the bottom strand. Termination signals for Pol III transcription are highlighted. Note spacer length heterogeneity.

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Open Data

Additional file 4:

Bisulfite analysis of the 5 S rDNA genic region(central part). Description: CyMATE program outputs from sequencing of non coding strands are shown. Filled symbols – methylated Cs; empty symbols non-methylated Cs. The numbers below the diagrams indicate C residues in the alignments. Gaps in matrices were caused by sequence polymorphisms.

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Open Data